@article{87a32a5e18ad47c891f2f6d4892e4542,
title = "Saposin B Binds the Lipofuscin Bisretinoid A2E and Prevents its Enzymatic and Photooxidation",
abstract = "Vitamin A based bisretinoid accumulation is a major focus in the study of macular degeneration. Whether specific endogenous lysosomal proteins can bind A2E, a pronounced bisretinoid in lipofuscin granules in retinal pigment epithelial cells, and interfere with enzymatic or photoinduced oxidation of such, has not been explored. Herein, using fluorescence and electronic absorption spectroscopy and mass spectrometry, we demonstrate that Saposin B, a critical protein in the degradation of sulfatides and “flushing” of lipids, can bind A2E, preventing its H2O2-dependent enzymatic oxidation by horseradish peroxidase and photooxidation by blue light (λ=450–460 nm).",
keywords = "bisretinoids, enzymatic oxidation, macular degeneration, photooxidation, saposin B",
author = "Jay Tinklepaugh and Smith, {Britannia M.} and Yan Nie and Kelsey Moody and Kris Grohn and Fadi Bou-Abdallah and Doyle, {Robert P.}",
note = "Funding Information: This work is supported by funding from New York State through the Center for Advanced Systems and Engineering- a NYSTAR designated center at Syracuse University, the National Science Foundation (NSF IGERT), Ichor Therapeutics (Lafayette, NY, US) (R.P.D)), and, in-part by, the Cottrell College Science Award (ID # 7892) from Research Corporation, and the National Institute of General Medical Sciences of the National Institutes of Health Award R15GM104879 (F.B.A.). Funding Information: . This work is supported by funding from New York State through the Center for Advanced Systems and Engineering‐ a NYSTAR designated center at Syracuse University, the National Science Foundation (NSF IGERT), Ichor Therapeutics (Lafayette, NY, US) (R.P.D)), and, in‐part by, the Cottrell College Science Award (ID # 7892) from Research Corporation, and the National Institute of General Medical Sciences of the National Institutes of Health Award R15GM104879 (F.B.A.) Publisher Copyright: {\textcopyright} 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim",
year = "2017",
month = jun,
day = "1",
doi = "10.1002/cptc.201700039",
language = "English (US)",
volume = "1",
pages = "256--259",
journal = "ChemPhotoChem",
issn = "2367-0932",
publisher = "Wiley-VCH Verlag",
number = "6",
}