Roles of NCX and PMCA in basolateral calcium export associated with mineralization cycles and cold acclimation in crayfish

M. G. Wheatly, Y. Gao, L. M. Stiner, D. R. Whalen, M. Nade, F. Vigo, A. E. Golshani

Research output: Chapter in Book/Report/Conference proceedingConference contribution

7 Scopus citations

Abstract

Basolateral Na+/Ca2+ exchanger (NCX) and plasma membrane Ca2+ ATPase (PMCA) are the primary transmembrane proteins that export calcium (Ca2+) from cells. In our lab we use a nonmammalian animal model, the freshwater crayfish, to study cellular Ca 2+ regulation. Two experimental conditions are employed to effect Ca2+ dyshomeostasis: (a) in the postmolt stage of the crustacean molting cycle increased unidirectional Ca2+ influx associated with cuticular mineralization is accompanied by elevated basolateral Ca2+ export (compared with intermolt Ca balance); and (b) exposure of the poikilothermic crayfish to cold acclimation (4°C) causes influx of Ca 2+ into cells, which is compensated by increased basolateral Ca 2+ export (compared with exposure to 23°C). This study compares expression of both NCX and PMCA mRNA (real-time PCR) and protein (Western) in both epithelial (kidney) and nonepithelial tissue (tail muscle) during elevated basolateral Ca2+ export. Both experimental treatments produced increases in NCX and PMCA expression (mRNA and protein) in both tissues. Mineralization produced greater upregulation of mRNA in kidney than in tail, whereas cold acclimation yielded comparable increases in both tissues. Protein expression patterns were generally confirmatory of real-time PCR data although expression changes were less pronounced. Both experimental treatments appear to increase basolateral Ca2+ export.

Original languageEnglish (US)
Title of host publicationSodium-Calcium Exchange and the Plasma Membrane Ca2+ATPase in Ce;; Funcyion
Subtitle of host publicationFifth International Conference
PublisherBlackwell Publishing Inc.
Pages190-192
Number of pages3
ISBN (Print)1573316490, 9781573316491
DOIs
StatePublished - Mar 2007
Externally publishedYes

Publication series

NameAnnals of the New York Academy of Sciences
Volume1099
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Keywords

  • Cold acclimation
  • Crayfish
  • Mineralization cycle
  • NCX
  • PMCA

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Fingerprint Dive into the research topics of 'Roles of NCX and PMCA in basolateral calcium export associated with mineralization cycles and cold acclimation in crayfish'. Together they form a unique fingerprint.

  • Cite this

    Wheatly, M. G., Gao, Y., Stiner, L. M., Whalen, D. R., Nade, M., Vigo, F., & Golshani, A. E. (2007). Roles of NCX and PMCA in basolateral calcium export associated with mineralization cycles and cold acclimation in crayfish. In Sodium-Calcium Exchange and the Plasma Membrane Ca2+ATPase in Ce;; Funcyion: Fifth International Conference (pp. 190-192). (Annals of the New York Academy of Sciences; Vol. 1099). Blackwell Publishing Inc.. https://doi.org/10.1196/annals.1387.022