RNAi as a tool for understanding germline development in Caenorhabditis elegans: Uses and cautions

Research output: Contribution to journalReview article

22 Scopus citations

Abstract

RNA-mediated genetic interference (RNAi) has become a very useful tool for analyzing gene function in development and other processes. RNAi can be used as a complement to traditional genetic studies or as a primary means of determining biological function. However, the efficacy of RNAi depends on a variety of factors that the researcher must take into consideration. This review focuses on germline development in the nematode, Caenorhabditis elegans, and discusses the uses and limitations of RNAi in providing new information about gene function as well as the possible endogenous role RNAi plays in germline physiology.

Original languageEnglish (US)
Pages (from-to)177-189
Number of pages13
JournalDevelopmental Biology
Volume239
Issue number2
DOIs
StatePublished - Nov 15 2001

Keywords

  • C. elegans
  • Caenorhabditis elegans
  • Development
  • Germ cells
  • Germ line
  • Germline development
  • RNA interference
  • RNAi

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'RNAi as a tool for understanding germline development in Caenorhabditis elegans: Uses and cautions'. Together they form a unique fingerprint.

  • Cite this