Ribosomal protein genes are highly enriched among genes with allele-specific expression in the interspecific F1 hybrid catfish

Ailu Chen, Ruijia Wang, Shikai Liu, Eric Peatman, Luyang Sun, Lisui Bao, Chen Jiang, Chao Li, Yun Li, Qifan Zeng, Zhanjian "John" Liu

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Interspecific hybrids provide a rich source for the analysis of allele-specific expression (ASE). In this work, we analyzed ASE in F1 hybrid catfish using RNA-Seq datasets. While the vast majority of genes were expressed with both alleles, 7–8 % SNPs exhibited significant differences in allele ratios of expression. Of the 66,251 and 177,841 SNPs identified from the datasets of the liver and gill, 5420 (8.2 %) and 13,390 (7.5 %) SNPs were identified as significant ASE-SNPs, respectively. With these SNPs, a total of 1519 and 3075 ASE-genes were identified. Gene Ontology analysis revealed that genes encoding cytoplasmic ribosomal proteins (RP) were highly enriched among ASE genes. Parent-of-origin was determined for 27 and 30 ASE RP genes in the liver and gill, respectively. The results indicated that genes from both channel catfish and blue catfish were involved in ASE. However, each RP gene appeared to be almost exclusively expressed from only one parent, indicating that ribosomes in the hybrid catfish were in the “hybrid” form. Overall representation of RP transcripts among the transcriptome appeared lower in the F1 hybrid catfish than in channel catfish or blue catfish, suggesting that the “hybrid” ribosomes may work more efficiently for translation in the F1 hybrid catfish.

Original languageEnglish (US)
Pages (from-to)1083-1093
Number of pages11
JournalMolecular Genetics and Genomics
Volume291
Issue number3
DOIs
StatePublished - Jun 1 2016
Externally publishedYes

Keywords

  • Allele-specific expression
  • ASE
  • Catfish
  • Fish
  • Genome
  • Hybrid
  • Ribosomal protein

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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