TY - JOUR
T1 - REVERSIBLE BLEACHING OF Chlamydomonas reinhardtii RHODOPSIN in vivo
AU - Hegemann, P.
AU - Hegemann, U.
AU - Foster, K. W.
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1988/7
Y1 - 1988/7
N2 - Abstract— The effect of hydroxylamine on the phototactic activity of Chlamydomonas reinhardtii was investigated. The following results were obtained: (1) wild type cells, irradiated for 10 min with green light immediately after addition of 1 mM hydroxylamine, showed a 20 min transient loss of phototactic activity, (2) irradiation of cells, preincubated in the dark with 4 mM. hydroxylamine for 30 min, diminished the phototactic sensitivity permanently by more than 100‐fold without loss of cell motility. (3) The phototactic sensitivity completely recovered within 3(1 min of the removal of hydroxylamin from carotenoid‐containing cells or from carotenoid‐negative cells upon addition of 11‐cis or all‐trans retinal. Our explanation is bleaching of rhodopsin by more than 99% and reconstitution by de novo synthesized or by added retinal.
AB - Abstract— The effect of hydroxylamine on the phototactic activity of Chlamydomonas reinhardtii was investigated. The following results were obtained: (1) wild type cells, irradiated for 10 min with green light immediately after addition of 1 mM hydroxylamine, showed a 20 min transient loss of phototactic activity, (2) irradiation of cells, preincubated in the dark with 4 mM. hydroxylamine for 30 min, diminished the phototactic sensitivity permanently by more than 100‐fold without loss of cell motility. (3) The phototactic sensitivity completely recovered within 3(1 min of the removal of hydroxylamin from carotenoid‐containing cells or from carotenoid‐negative cells upon addition of 11‐cis or all‐trans retinal. Our explanation is bleaching of rhodopsin by more than 99% and reconstitution by de novo synthesized or by added retinal.
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U2 - 10.1111/j.1751-1097.1988.tb02796.x
DO - 10.1111/j.1751-1097.1988.tb02796.x
M3 - Article
C2 - 3217436
AN - SCOPUS:0024048990
SN - 0031-8655
VL - 48
SP - 123
EP - 128
JO - Photochemistry and photobiology
JF - Photochemistry and photobiology
IS - 1
ER -