REVERSIBLE BLEACHING OF Chlamydomonas reinhardtii RHODOPSIN in vivo

P. Hegemann, U. Hegemann, K. W. Foster

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

Abstract— The effect of hydroxylamine on the phototactic activity of Chlamydomonas reinhardtii was investigated. The following results were obtained: (1) wild type cells, irradiated for 10 min with green light immediately after addition of 1 mM hydroxylamine, showed a 20 min transient loss of phototactic activity, (2) irradiation of cells, preincubated in the dark with 4 mM. hydroxylamine for 30 min, diminished the phototactic sensitivity permanently by more than 100‐fold without loss of cell motility. (3) The phototactic sensitivity completely recovered within 3(1 min of the removal of hydroxylamin from carotenoid‐containing cells or from carotenoid‐negative cells upon addition of 11‐cis or all‐trans retinal. Our explanation is bleaching of rhodopsin by more than 99% and reconstitution by de novo synthesized or by added retinal.

Original languageEnglish (US)
Pages (from-to)123-128
Number of pages6
JournalPhotochemistry and Photobiology
Volume48
Issue number1
DOIs
StatePublished - Jul 1988

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry

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