Resolving mechanisms of competitive fertilization success in drosophila Melanogaster

Mollie K. Manier, John M. Belote, Kirstin S. Berben, David Novikov, Will T. Stuart, Scott Pitnick

Research output: Contribution to journalArticle

188 Scopus citations

Abstract

Our understanding of postcopulatory sexual selection has been constrained by an inability to discriminate competing sperm of different males, coupled with challenges of directly observing live sperm inside the female reproductive tract. Real-time and spatiotemporal analyses of sperm movement, storage, and use within female Drosophila melanogaster inseminated by two transgenic males with, respectively, green and red sperm heads allowed us to unambiguously discriminate among hypothesized mechanisms underlying sperm precedence, including physical displacement and incapacitation of "resident" sperm by second males, female ejection of sperm, and biased use of competing sperm for fertilization. We find that competitive male fertilization success derives from a multivariate process involving ejaculate-female and ejaculate-ejaculate interactions, as well as complex sperm behavior in vivo.

Original languageEnglish (US)
Pages (from-to)354-357
Number of pages4
JournalScience
Volume328
Issue number5976
DOIs
StatePublished - Apr 16 2010

ASJC Scopus subject areas

  • General

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    Manier, M. K., Belote, J. M., Berben, K. S., Novikov, D., Stuart, W. T., & Pitnick, S. (2010). Resolving mechanisms of competitive fertilization success in drosophila Melanogaster. Science, 328(5976), 354-357. https://doi.org/10.1126/science.1187096