Reinvestigation of coenzyme Q10 isolation from sporidiobolus johnsonii

David D. Dixson, Christopher N. Boddy, Robert Patrick Doyle

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

There is considerable current interest in coenzyme Q10 (CoQ10) from a medical perspective. CoQ10 has been shown to alleviate the side effects of statin drugs, for instance, and so there is a push to find naturally high producers of the compound. Sporidiobolus johnsonii (S. johnsonii) has been reported to produce CoQ10 in studies that used only standards on thin-layer chromatography (TLC) and also suggested the production of coenzyme Q9 (CoQ9). This work set out to verify CoQ9/CoQ10 production in S. johnsonii and quantify as appropriate. We show that S. johnsonii produces CoQ10 but found no evidence for CoQ9 biosynthesis. The specific production of CoQ10 was noted at 10 mg/g dry cell weight (DCW) in media supplemented with 4-hydroxybenzoic acid (HBA). This makes S. johnsonii a naturally high CoQ10 producer. New methods for extraction and purification of CoQ10 are also discussed, and identification of a closely eluting side product under normal phase isolation is reported.

Original languageEnglish (US)
Pages (from-to)1033-1051
Number of pages19
JournalChemistry and Biodiversity
Volume8
Issue number6
DOIs
StatePublished - Jun 2011

Fingerprint

coenzyme Q10
Coenzymes
Hydroxymethylglutaryl-CoA Reductase Inhibitors
Thin layer chromatography
Biosynthesis
Thin Layer Chromatography
Drug-Related Side Effects and Adverse Reactions

Keywords

  • Benzoic acid, 4-hydroxy-
  • Coenzyme Q10
  • Fermentation
  • Sporidiobolus johnsonii

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Bioengineering
  • Molecular Biology
  • Molecular Medicine

Cite this

Reinvestigation of coenzyme Q10 isolation from sporidiobolus johnsonii. / Dixson, David D.; Boddy, Christopher N.; Doyle, Robert Patrick.

In: Chemistry and Biodiversity, Vol. 8, No. 6, 06.2011, p. 1033-1051.

Research output: Contribution to journalArticle

Dixson, David D. ; Boddy, Christopher N. ; Doyle, Robert Patrick. / Reinvestigation of coenzyme Q10 isolation from sporidiobolus johnsonii. In: Chemistry and Biodiversity. 2011 ; Vol. 8, No. 6. pp. 1033-1051.
@article{bd292eaa2aac495f924690b9a720e7b5,
title = "Reinvestigation of coenzyme Q10 isolation from sporidiobolus johnsonii",
abstract = "There is considerable current interest in coenzyme Q10 (CoQ10) from a medical perspective. CoQ10 has been shown to alleviate the side effects of statin drugs, for instance, and so there is a push to find naturally high producers of the compound. Sporidiobolus johnsonii (S. johnsonii) has been reported to produce CoQ10 in studies that used only standards on thin-layer chromatography (TLC) and also suggested the production of coenzyme Q9 (CoQ9). This work set out to verify CoQ9/CoQ10 production in S. johnsonii and quantify as appropriate. We show that S. johnsonii produces CoQ10 but found no evidence for CoQ9 biosynthesis. The specific production of CoQ10 was noted at 10 mg/g dry cell weight (DCW) in media supplemented with 4-hydroxybenzoic acid (HBA). This makes S. johnsonii a naturally high CoQ10 producer. New methods for extraction and purification of CoQ10 are also discussed, and identification of a closely eluting side product under normal phase isolation is reported.",
keywords = "Benzoic acid, 4-hydroxy-, Coenzyme Q10, Fermentation, Sporidiobolus johnsonii",
author = "Dixson, {David D.} and Boddy, {Christopher N.} and Doyle, {Robert Patrick}",
year = "2011",
month = "6",
doi = "10.1002/cbdv.201000278",
language = "English (US)",
volume = "8",
pages = "1033--1051",
journal = "Chemistry and Biodiversity",
issn = "1612-1872",
publisher = "Wiley-VCH Verlag",
number = "6",

}

TY - JOUR

T1 - Reinvestigation of coenzyme Q10 isolation from sporidiobolus johnsonii

AU - Dixson, David D.

AU - Boddy, Christopher N.

AU - Doyle, Robert Patrick

PY - 2011/6

Y1 - 2011/6

N2 - There is considerable current interest in coenzyme Q10 (CoQ10) from a medical perspective. CoQ10 has been shown to alleviate the side effects of statin drugs, for instance, and so there is a push to find naturally high producers of the compound. Sporidiobolus johnsonii (S. johnsonii) has been reported to produce CoQ10 in studies that used only standards on thin-layer chromatography (TLC) and also suggested the production of coenzyme Q9 (CoQ9). This work set out to verify CoQ9/CoQ10 production in S. johnsonii and quantify as appropriate. We show that S. johnsonii produces CoQ10 but found no evidence for CoQ9 biosynthesis. The specific production of CoQ10 was noted at 10 mg/g dry cell weight (DCW) in media supplemented with 4-hydroxybenzoic acid (HBA). This makes S. johnsonii a naturally high CoQ10 producer. New methods for extraction and purification of CoQ10 are also discussed, and identification of a closely eluting side product under normal phase isolation is reported.

AB - There is considerable current interest in coenzyme Q10 (CoQ10) from a medical perspective. CoQ10 has been shown to alleviate the side effects of statin drugs, for instance, and so there is a push to find naturally high producers of the compound. Sporidiobolus johnsonii (S. johnsonii) has been reported to produce CoQ10 in studies that used only standards on thin-layer chromatography (TLC) and also suggested the production of coenzyme Q9 (CoQ9). This work set out to verify CoQ9/CoQ10 production in S. johnsonii and quantify as appropriate. We show that S. johnsonii produces CoQ10 but found no evidence for CoQ9 biosynthesis. The specific production of CoQ10 was noted at 10 mg/g dry cell weight (DCW) in media supplemented with 4-hydroxybenzoic acid (HBA). This makes S. johnsonii a naturally high CoQ10 producer. New methods for extraction and purification of CoQ10 are also discussed, and identification of a closely eluting side product under normal phase isolation is reported.

KW - Benzoic acid, 4-hydroxy-

KW - Coenzyme Q10

KW - Fermentation

KW - Sporidiobolus johnsonii

UR - http://www.scopus.com/inward/record.url?scp=79959240209&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79959240209&partnerID=8YFLogxK

U2 - 10.1002/cbdv.201000278

DO - 10.1002/cbdv.201000278

M3 - Article

C2 - 21674778

AN - SCOPUS:79959240209

VL - 8

SP - 1033

EP - 1051

JO - Chemistry and Biodiversity

JF - Chemistry and Biodiversity

SN - 1612-1872

IS - 6

ER -