Regional differences in expression of VEGF mRNA in rat gastrocnemiusfollowing 1 hr exercise or electrical stimulation

Tom D. Brutsaert, Timothy P. Gavin, Zhenxing Fu, Ellen C. Breen, Kechun Tang, Odile Mathieu-Costello, Peter D. Wagner

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Background: Vascular endothelial growth factor (VEGF) mRNA levels increase in rat skeletal muscle after a single bout of acute exercise. We assessed regional differences in VEGF165 mRNA levels in rat gastrocnemius muscle using in situ hybridization after inducing upregulation of VEGF by treadmill running (1 hr) or electrical stimulation (1 hr). Muscle functional regions were defined as oxidative (primarily oxidative fibers, I and IIa), or glycolytic (entirely IIb or IId/x fibers). Functional regions were visualized on muscle cross sections that were matched in series to slides processed through in situ (hybridization with a VEGF165 probe. A greater upregulation in oxidative regions was hypothesized. Results: Total muscle VEGF mRNA (via Northern blot) was upregulated 3.5-fold with both exercise and with electrical stimulation (P = 0.015). Quantitative densitometry of the VEGF mRNA signal via in situ hybridization reveals significant regional differences (P ≤ 0.01) and protocol (differences (treadmill, electrical stimulation, and control, P ≤ 0.05). Mean VEGF mRNA signal was (higher in the oxidative region in both treadmill run (∼7%, N = 4 muscles, P ≤ 0.05) and electrically stimulated muscles (∼60%, N = 4, (P ≤ 0.05). These regional differences were not significantly different from control muscle (non-exercised, non-stimulated, N = 2 muscles), although nearly so for electrically stimulated muscle (P = 0.056). Conclusions: Moderately higher VEGF mRNA signal in oxidative muscle regions is consistent with regional differences in capillary density. However, it is not possible to determine if the VEGF mRNA signal difference is important in either the maintenance of regional capillarity differences or exercise induced angiogenesis.

Original languageEnglish (US)
Article number1
Pages (from-to)1-10
Number of pages10
JournalBMC Physiology
Volume2
DOIs
StatePublished - Jun 19 2002

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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