TY - GEN
T1 - Reconstitution of human primordial germ cell development from pluripotent stem cells in a synthetic embryonic environment
AU - Esfahani, Sajedeh Nasr
AU - Zheng, Yi
AU - Resto Irizarry, Agnes M.
AU - Shao, Yue
AU - Wang, Sicong
AU - Xue, Xufeng
AU - Fu, Jianping
N1 - Publisher Copyright:
© 2020 CBMS-0001
PY - 2020
Y1 - 2020
N2 - Primordial germ cells (PGCs) are the precursors of sperm and eggs, which generate the totipotent state. Without a tractable experimental model, the mechanism of human PGC (hPGC) specification remains unclear. We report the induction of hPGC-like cells (hPGCLCs) directly from primed human pluripotent stem cells (hPSCs) in a synthetic embryonic environment recapitulating the in vivo niche of the posterior end of the primate embryonic sac. We demonstrated that amniotic ectoderm-like cells (AMLCs) trigger the specification of PGCLCs from primed hPSCs. Importantly, using this synthetic system, we, for the first time, elucidate the important role of ACTIVIN-NODAL signaling in hPGCLC specification.
AB - Primordial germ cells (PGCs) are the precursors of sperm and eggs, which generate the totipotent state. Without a tractable experimental model, the mechanism of human PGC (hPGC) specification remains unclear. We report the induction of hPGC-like cells (hPGCLCs) directly from primed human pluripotent stem cells (hPSCs) in a synthetic embryonic environment recapitulating the in vivo niche of the posterior end of the primate embryonic sac. We demonstrated that amniotic ectoderm-like cells (AMLCs) trigger the specification of PGCLCs from primed hPSCs. Importantly, using this synthetic system, we, for the first time, elucidate the important role of ACTIVIN-NODAL signaling in hPGCLC specification.
KW - Amniogenesis
KW - Human Pluripotent Stem Cell
KW - Human development
KW - Primordial Germ Cell
UR - http://www.scopus.com/inward/record.url?scp=85098287492&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85098287492&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85098287492
T3 - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 420
EP - 421
BT - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
Y2 - 4 October 2020 through 9 October 2020
ER -