Reconstitution of human primordial germ cell development from pluripotent stem cells in a synthetic embryonic environment

Sajedeh Nasr Esfahani, Yi Zheng, Agnes M. Resto Irizarry, Yue Shao, Sicong Wang, Xufeng Xue, Jianping Fu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Primordial germ cells (PGCs) are the precursors of sperm and eggs, which generate the totipotent state. Without a tractable experimental model, the mechanism of human PGC (hPGC) specification remains unclear. We report the induction of hPGC-like cells (hPGCLCs) directly from primed human pluripotent stem cells (hPSCs) in a synthetic embryonic environment recapitulating the in vivo niche of the posterior end of the primate embryonic sac. We demonstrated that amniotic ectoderm-like cells (AMLCs) trigger the specification of PGCLCs from primed hPSCs. Importantly, using this synthetic system, we, for the first time, elucidate the important role of ACTIVIN-NODAL signaling in hPGCLC specification.

Original languageEnglish (US)
Title of host publicationMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages420-421
Number of pages2
ISBN (Electronic)9781733419017
StatePublished - 2020
Externally publishedYes
Event24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 - Virtual, Online
Duration: Oct 4 2020Oct 9 2020

Publication series

NameMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
CityVirtual, Online
Period10/4/2010/9/20

Keywords

  • Amniogenesis
  • Human Pluripotent Stem Cell
  • Human development
  • Primordial Germ Cell

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering
  • Chemistry(all)
  • Control and Systems Engineering

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