Rate enhancements in the DNase I footprinting experiment.

B. Ward, R. Rehfuss, J. Goodisman, J. C. Dabrowiak

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Footprinting experiments for DNase I digests of a 139-base-pair segment of pBR-322 DNA in the presence of either netropsin or actinomycin D were carried out. Plots of oligonucleotide concentration as a function of drug concentration were analyzed to study the enhancement in cleavage rates at approximately 30 sites, accompanying drug binding at other sites. The pattern of enhancements is not consistent with drug-induced DNA structural changes, but agrees with a redistribution mechanism involving DNase I. Since the total number of enzyme molecules per fragment remains unchanged, drug binding at some sites increases the enzyme concentration at other sites, giving rise to increased cleavage. The consequences of the redistribution mechanism for analysis of footprinting experiments are indicated.

Original languageEnglish (US)
Pages (from-to)1359-1369
Number of pages11
JournalNucleic acids research
Volume16
Issue number4
StatePublished - Feb 25 1988

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Rate enhancements in the DNase I footprinting experiment.'. Together they form a unique fingerprint.

  • Cite this

    Ward, B., Rehfuss, R., Goodisman, J., & Dabrowiak, J. C. (1988). Rate enhancements in the DNase I footprinting experiment. Nucleic acids research, 16(4), 1359-1369.