Rapid measurement of specific membrane capacitance and cytoplasm conductivity on single cells

Yi Zheng; Ehsan Shojaei-Baghini; Chen Wang; Yu Sun

doi:10.1109/MEMSYS.2013.6474443

Rapid measurement of specific membrane capacitance and cytoplasm conductivity on single cells

Yi Zheng, Ehsan Shojaei-Baghini, Chen Wang, Yu Sun

Research output: Chapter in Book/Entry/Poem › Conference contribution

3 Scopus citations

Abstract

This paper presents a technique for the characterization of single-cell electrical properties (specific membrane capacitance and cytoplasm conductivity) at a speed of 5-10 cells per second (vs. minutes per cell using existing techniques). When a cell flows through a microfluidic constriction channel, electrical impedance at multiple frequencies is measured. Electrical and geometrical models were developed to interpret the impedance data and determine the specific membrane capacitance and cytoplasm conductivity of individual cells. Results from testing 3,249 AML-2 cells and 3,398 HL-60 cells reveal different electrical properties between these two cell types. The results also demonstrate that the quantification of specific membrane capacitance and cytoplasm conductivity can enhance cell classification results.

Original language	English (US)
Title of host publication	IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
Pages	1105-1108
Number of pages	4
DOIs	https://doi.org/10.1109/MEMSYS.2013.6474443
State	Published - 2013
Externally published	Yes
Event	IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 - Taipei, Taiwan, Province of China Duration: Jan 20 2013 → Jan 24 2013

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Conference

Conference	IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
Country/Territory	Taiwan, Province of China
City	Taipei
Period	1/20/13 → 1/24/13

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

Access to Document

10.1109/MEMSYS.2013.6474443

Cite this

Zheng, Y., Shojaei-Baghini, E., Wang, C., & Sun, Y. (2013). Rapid measurement of specific membrane capacitance and cytoplasm conductivity on single cells. In IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 (pp. 1105-1108). Article 6474443 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2013.6474443

Rapid measurement of specific membrane capacitance and cytoplasm conductivity on single cells. / Zheng, Yi; Shojaei-Baghini, Ehsan; Wang, Chen et al.
IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013. 2013. p. 1105-1108 6474443 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Entry/Poem › Conference contribution

Zheng, Y, Shojaei-Baghini, E, Wang, C & Sun, Y 2013, Rapid measurement of specific membrane capacitance and cytoplasm conductivity on single cells. in IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013., 6474443, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 1105-1108, IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013, Taipei, Taiwan, Province of China, 1/20/13. https://doi.org/10.1109/MEMSYS.2013.6474443

Zheng Y, Shojaei-Baghini E, Wang C, Sun Y. Rapid measurement of specific membrane capacitance and cytoplasm conductivity on single cells. In IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013. 2013. p. 1105-1108. 6474443. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMSYS.2013.6474443

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title = "Rapid measurement of specific membrane capacitance and cytoplasm conductivity on single cells",

abstract = "This paper presents a technique for the characterization of single-cell electrical properties (specific membrane capacitance and cytoplasm conductivity) at a speed of 5-10 cells per second (vs. minutes per cell using existing techniques). When a cell flows through a microfluidic constriction channel, electrical impedance at multiple frequencies is measured. Electrical and geometrical models were developed to interpret the impedance data and determine the specific membrane capacitance and cytoplasm conductivity of individual cells. Results from testing 3,249 AML-2 cells and 3,398 HL-60 cells reveal different electrical properties between these two cell types. The results also demonstrate that the quantification of specific membrane capacitance and cytoplasm conductivity can enhance cell classification results.",

author = "Yi Zheng and Ehsan Shojaei-Baghini and Chen Wang and Yu Sun",

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doi = "10.1109/MEMSYS.2013.6474443",

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N2 - This paper presents a technique for the characterization of single-cell electrical properties (specific membrane capacitance and cytoplasm conductivity) at a speed of 5-10 cells per second (vs. minutes per cell using existing techniques). When a cell flows through a microfluidic constriction channel, electrical impedance at multiple frequencies is measured. Electrical and geometrical models were developed to interpret the impedance data and determine the specific membrane capacitance and cytoplasm conductivity of individual cells. Results from testing 3,249 AML-2 cells and 3,398 HL-60 cells reveal different electrical properties between these two cell types. The results also demonstrate that the quantification of specific membrane capacitance and cytoplasm conductivity can enhance cell classification results.

AB - This paper presents a technique for the characterization of single-cell electrical properties (specific membrane capacitance and cytoplasm conductivity) at a speed of 5-10 cells per second (vs. minutes per cell using existing techniques). When a cell flows through a microfluidic constriction channel, electrical impedance at multiple frequencies is measured. Electrical and geometrical models were developed to interpret the impedance data and determine the specific membrane capacitance and cytoplasm conductivity of individual cells. Results from testing 3,249 AML-2 cells and 3,398 HL-60 cells reveal different electrical properties between these two cell types. The results also demonstrate that the quantification of specific membrane capacitance and cytoplasm conductivity can enhance cell classification results.

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T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

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Rapid measurement of specific membrane capacitance and cytoplasm conductivity on single cells

Abstract

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