Rapid measurement of specific membrane capacitance and cytoplasm conductivity on single cells

Yi Zheng, Ehsan Shojaei-Baghini, Chen Wang, Yu Sun

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

This paper presents a technique for the characterization of single-cell electrical properties (specific membrane capacitance and cytoplasm conductivity) at a speed of 5-10 cells per second (vs. minutes per cell using existing techniques). When a cell flows through a microfluidic constriction channel, electrical impedance at multiple frequencies is measured. Electrical and geometrical models were developed to interpret the impedance data and determine the specific membrane capacitance and cytoplasm conductivity of individual cells. Results from testing 3,249 AML-2 cells and 3,398 HL-60 cells reveal different electrical properties between these two cell types. The results also demonstrate that the quantification of specific membrane capacitance and cytoplasm conductivity can enhance cell classification results.

Original languageEnglish (US)
Title of host publicationIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
Pages1105-1108
Number of pages4
DOIs
StatePublished - 2013
Externally publishedYes
EventIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 - Taipei, Taiwan, Province of China
Duration: Jan 20 2013Jan 24 2013

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Conference

ConferenceIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
Country/TerritoryTaiwan, Province of China
CityTaipei
Period1/20/131/24/13

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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