Raman spectroscopic investigation of spinal cord injury in a rat model

Tarun Saxena, Bin Deng, Dennis Stelzner, Julie M Hasenwinkel, Joseph Chaiken

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Raman spectroscopy was used to study temporal molecular changes associated with spinal cord injury (SCI) in a rat model. Raman spectra of saline-perfused, injured, and healthy rat spinal cords were obtained and compared. Two injury models, a lateral hemisection and a moderate contusion were investigated. The net fluorescence and the Raman spectra showed clear differences between the injured and healthy spinal cords. Based on extensive histological and biochemical characterization of SCI available in the literature, these differences were hypothesized to be due to cell death, demyelination, and changes in the extracellular matrix composition, such as increased expression of proteoglycans and hyaluronic acid, at the site of injury where the glial scar forms. Further, analysis of difference spectra indicated the presence of carbonyl containing compounds, hypothesized to be products of lipid peroxidation and acid catalyzed hydrolysis of glycosaminoglycan moieties. These results compared well with in vitro experiments conducted on chondroitin sulfate sugars. Since the glial scar is thought to be a potent biochemical barrier to nerve regeneration, this observation suggests the possibility of using near infrared Raman spectroscopy to study injury progression and explore potential treatments ex vivo, and ultimately monitor potential remedial treatments within the spinal cord in vivo.

Original languageEnglish (US)
Article number027003
JournalJournal of Biomedical Optics
Volume16
Issue number2
DOIs
StatePublished - Feb 2011

Fingerprint

spinal cord injuries
spinal cord
rats
Rats
scars
Raman spectroscopy
Raman spectra
acids
carbonyl compounds
nerves
Raman scattering
sugars
regeneration
death
progressions
lipids
hydrolysis
monitors
sulfates
Hyaluronic acid

Keywords

  • Chondroitin sulfate proteoglycans
  • Glial scar
  • Raman spectroscopy
  • Spinal cord injury

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Raman spectroscopic investigation of spinal cord injury in a rat model. / Saxena, Tarun; Deng, Bin; Stelzner, Dennis; Hasenwinkel, Julie M; Chaiken, Joseph.

In: Journal of Biomedical Optics, Vol. 16, No. 2, 027003, 02.2011.

Research output: Contribution to journalArticle

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