R161, K452 and R460 residues are vital for metal-citrate complex transport in CitSc from streptomyces coelicolor

Joshua J. Lensbouer, Qi Wen Li, Maggie Estlinbaum, Robert Patrick Doyle

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Recent discoveries have been made that demonstrate Gram-positive bacteria can transport metal-citrate complexes through the CitMHS family of proteins in symport with H+ ions. The CitMHS family of transporters investigated to date have the ability to selectively transport only certain metal-citrate complexes. Despite sharing amino acid sequence similarity as high as 73%; predicting what complexes are transported remains difficult. The iron-citrate transporter from Streptomyces coelicolor has been mutated at three postulated critical sites (R161, K452 and R460) based on activity modeling against the LacY permease. All three mutants eliminate or greatly reduce uptake of metal-citrate complexes tested. The implications of this are discussed.

Original languageEnglish (US)
Pages (from-to)342-347
Number of pages6
JournalMetallomics
Volume2
Issue number5
DOIs
StatePublished - 2010

Fingerprint

Streptomyces coelicolor
Coordination Complexes
Citric Acid
Metals
Membrane Transport Proteins
Ion Transport
Gram-Positive Bacteria
Amino acids
Amino Acid Sequence
Bacteria
Iron
Ions
Proteins
Amino Acids

ASJC Scopus subject areas

  • Biomaterials
  • Metals and Alloys
  • Chemistry (miscellaneous)
  • Biochemistry
  • Biophysics

Cite this

R161, K452 and R460 residues are vital for metal-citrate complex transport in CitSc from streptomyces coelicolor. / Lensbouer, Joshua J.; Li, Qi Wen; Estlinbaum, Maggie; Doyle, Robert Patrick.

In: Metallomics, Vol. 2, No. 5, 2010, p. 342-347.

Research output: Contribution to journalArticle

Lensbouer, Joshua J. ; Li, Qi Wen ; Estlinbaum, Maggie ; Doyle, Robert Patrick. / R161, K452 and R460 residues are vital for metal-citrate complex transport in CitSc from streptomyces coelicolor. In: Metallomics. 2010 ; Vol. 2, No. 5. pp. 342-347.
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