Quantitatively characterizing drug-induced arrhythmic contractile motions of human stem cell-derived cardiomyocytes

Plansky Hoang, Nathaniel Huebsch, Shin Hyuk Bang, Brian A. Siemons, Bruce R. Conklin, Kevin E. Healy, Zhen Ma, Sabir Jacquir

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Quantification of abnormal contractile motions of cardiac tissue has been a noteworthy challenge and significant limitation in assessing and classifying the drug-induced arrhythmias (i.e., Torsades de pointes). To overcome these challenges, researchers have taken advantage of computational image processing tools to measure contractile motion from cardiomyocytes derived from human induced pluripotent stem cells (hiPSC-CMs). However, the amplitude and frequency analysis of contractile motion waveforms does not produce sufficient information to objectively classify the degree of variations between two or more sets of cardiac contractile motions. In this paper, we generated contractile motion data from beating hiPSC-CMs using motion tracking software based on optical flow analysis, and then implemented a computational algorithm, phase space reconstruction (PSR), to derive parameters (embedding, regularity, and fractal dimensions) to further characterize the dynamic nature of the cardiac contractile motions. Application of drugs known to cause cardiac arrhythmia induced significant changes to these resultant dimensional parameters calculated from PSR analysis. Integrating this new computational algorithm with the existing analytical toolbox of cardiac contractile motions will allow us to expand current assessments of cardiac tissue physiology into an automated, high-throughput, and quantifiable manner which will allow more objective assessments of drug-induced proarrhythmias.

Original languageEnglish (US)
Pages (from-to)1958-1970
Number of pages13
JournalBiotechnology and Bioengineering
Issue number8
StatePublished - Aug 2018


  • arrhythmia
  • biosignal processing
  • cardiac motion
  • optical flow
  • phase space reconstruction

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


Dive into the research topics of 'Quantitatively characterizing drug-induced arrhythmic contractile motions of human stem cell-derived cardiomyocytes'. Together they form a unique fingerprint.

Cite this