Quantitative Footprinting Analysis. Binding to a Single Site

Robert Rehfuss, Jerry Goodisman, James C. Dabrowiak

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


The theory for measuring ligand binding constants from footprinting autoradiographic data associated with a single binding site is derived. If the ligand and DNA cleavage agent compete for a common site, the spot intensities are not proportional to the amount of DNA not blocked by ligand. The analysis of a single site is experimentally illustrated by using results for the anticancer drug actinomycin D interacting with the duplex d(TAGCGCTA)2 as probed with the hydrolytic enzyme DNase I.

Original languageEnglish (US)
Pages (from-to)777-781
Number of pages5
Issue number3
StatePublished - Jan 1 1990

ASJC Scopus subject areas

  • Biochemistry


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