Quantification of the kinetics and thermodynamics of protein adsorption using atomic force microscopy

R. Gettens, J. L. Gilbert, Z. Bai

Research output: Chapter in Book/Report/Conference proceedingConference contribution


A set of rules on specific material properties that effect protein adsorption and conformation to device surfaces was developed. The protein used was bovine plasma fibrinogen in a pH 7.4 phosphate buffered saline solute and the substrate used was Grade IV muscovite mica. It was observed that the bolus method, one of the methods used in the study, gave superior imaging resolution while the sample was in-situ. It was suggested that by using the fluid flow method, it was possible to obtain real time kinetic data while being able to introduce the shear effects that would be present in blood contacting devices in the circulatory system.

Original languageEnglish (US)
Title of host publicationTransactions - 7th World Biomaterials Congress
Number of pages1
StatePublished - Dec 1 2004
EventTransactions - 7th World Biomaterials Congress - Sydney, Australia
Duration: May 17 2004May 21 2004

Publication series

NameTransactions - 7th World Biomaterials Congress


OtherTransactions - 7th World Biomaterials Congress

ASJC Scopus subject areas

  • Engineering(all)


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