Putative SNP discovery in interspecific hybrids of catfish by comparative EST analysis

C. He, L. Chen, M. Simmons, P. Li, S. Kim, Z. J. Liu

Research output: Contribution to journalArticle

73 Scopus citations

Abstract

In this study, we identified putative SNP markers within genes by comparative analysis of expressed sequence tags (ESTs). Comparison of 849 ESTs from blue catfish (Ictalurus furcatus) with >11 000 ESTs from channel catfish (I. punctatus) deposited in GenBank resulted in the identification of 1020 putative SNPs within 161 genes, of which 145 were nuclear genes of known function. The observed frequency of SNPs within ESTs of the two closely related catfish species was 1.32 SNP per 100 bp. The majority of identified SNPs differed between the two species and, therefore, these SNPs are useful for mapping genes in channel catfish x blue catfish interspecific resource families. The SNPs that differed within species were also observed; these can be applied to genome scans in channel catfish resource families.

Original languageEnglish (US)
Pages (from-to)445-448
Number of pages4
JournalAnimal Genetics
Volume34
Issue number6
DOIs
StatePublished - Dec 1 2003
Externally publishedYes

Keywords

  • Aquaculture
  • Expressed sequence tag
  • Fish
  • Marker
  • Single nucleotide polymorphism

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Genetics

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