Purification of vitellin from the ovary of Chinese mitten-handed crab (Eriocheir sinensis) and development of an antivitellin ELISA

Liqiao Chen; Hongbo Jiang; Zhongliang Zhou; Kang Li; Kai Li; Glenn Y. Deng; Zhanjiang Liu

doi:10.1016/j.cbpc.2004.04.012

Purification of vitellin from the ovary of Chinese mitten-handed crab (Eriocheir sinensis) and development of an antivitellin ELISA

Liqiao Chen, Hongbo Jiang, Zhongliang Zhou, Kang Li, Kai Li, Glenn Y. Deng, Zhanjiang Liu

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

Vitellin was purified from ovaries of mature female Chinese mitten-handed crab (Eriocheir sinensis) using gel filtration chromatography. Analysis by native PAGE showed the vitellin had a native molecular mass of 520 kDa, while denaturing SDS-PAGE revealed two subunits of 97 and 74 kDa. Purified vitellin was used to raise polyclonal antisera, with which an enzyme-linked immunosorbent assay (ELISA) was developed. The ELISA was sensitive and could effectively detect vitellin in the range of 7.8-500 ng. Furthermore, vitellin levels in various developmental stages of oogenesis were measured with the ELISA assay. The results indicated that levels of vitellin increased significantly from 0.22 mg/ovary at Stage II to 360.31 mg/ovary at Stage IV.

Original language	English (US)
Pages (from-to)	305-311
Number of pages	7
Journal	Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume	138
Issue number	3
DOIs	https://doi.org/10.1016/j.cbpc.2004.04.012
State	Published - Jul 2004
Externally published	Yes

Keywords

Decapoda
Developmental stages
Enzyme-linked immunosorbent assay
Eriocheir sinensis
Gel filtration chromatography
Ovary
Purification
Vitellin

ASJC Scopus subject areas

Aquatic Science
Animal Science and Zoology
Molecular Biology
Biochemistry
Physiology

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10.1016/j.cbpc.2004.04.012

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Purification of vitellin from the ovary of Chinese mitten-handed crab (Eriocheir sinensis) and development of an antivitellin ELISA. / Chen, Liqiao; Jiang, Hongbo; Zhou, Zhongliang et al.
In: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, Vol. 138, No. 3, 07.2004, p. 305-311.

Research output: Contribution to journal › Article › peer-review

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title = "Purification of vitellin from the ovary of Chinese mitten-handed crab (Eriocheir sinensis) and development of an antivitellin ELISA",

abstract = "Vitellin was purified from ovaries of mature female Chinese mitten-handed crab (Eriocheir sinensis) using gel filtration chromatography. Analysis by native PAGE showed the vitellin had a native molecular mass of 520 kDa, while denaturing SDS-PAGE revealed two subunits of 97 and 74 kDa. Purified vitellin was used to raise polyclonal antisera, with which an enzyme-linked immunosorbent assay (ELISA) was developed. The ELISA was sensitive and could effectively detect vitellin in the range of 7.8-500 ng. Furthermore, vitellin levels in various developmental stages of oogenesis were measured with the ELISA assay. The results indicated that levels of vitellin increased significantly from 0.22 mg/ovary at Stage II to 360.31 mg/ovary at Stage IV.",

keywords = "Decapoda, Developmental stages, Enzyme-linked immunosorbent assay, Eriocheir sinensis, Gel filtration chromatography, Ovary, Purification, Vitellin",

author = "Liqiao Chen and Hongbo Jiang and Zhongliang Zhou and Kang Li and Kai Li and Deng, {Glenn Y.} and Zhanjiang Liu",

note = "Funding Information: This study was supported by grants 30271012, 30130040 and 3028018 from the National Nature Science Foundation of China.",

year = "2004",

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doi = "10.1016/j.cbpc.2004.04.012",

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T1 - Purification of vitellin from the ovary of Chinese mitten-handed crab (Eriocheir sinensis) and development of an antivitellin ELISA

AU - Chen, Liqiao

AU - Jiang, Hongbo

AU - Zhou, Zhongliang

AU - Li, Kang

AU - Li, Kai

AU - Deng, Glenn Y.

AU - Liu, Zhanjiang

N1 - Funding Information: This study was supported by grants 30271012, 30130040 and 3028018 from the National Nature Science Foundation of China.

PY - 2004/7

Y1 - 2004/7

N2 - Vitellin was purified from ovaries of mature female Chinese mitten-handed crab (Eriocheir sinensis) using gel filtration chromatography. Analysis by native PAGE showed the vitellin had a native molecular mass of 520 kDa, while denaturing SDS-PAGE revealed two subunits of 97 and 74 kDa. Purified vitellin was used to raise polyclonal antisera, with which an enzyme-linked immunosorbent assay (ELISA) was developed. The ELISA was sensitive and could effectively detect vitellin in the range of 7.8-500 ng. Furthermore, vitellin levels in various developmental stages of oogenesis were measured with the ELISA assay. The results indicated that levels of vitellin increased significantly from 0.22 mg/ovary at Stage II to 360.31 mg/ovary at Stage IV.

AB - Vitellin was purified from ovaries of mature female Chinese mitten-handed crab (Eriocheir sinensis) using gel filtration chromatography. Analysis by native PAGE showed the vitellin had a native molecular mass of 520 kDa, while denaturing SDS-PAGE revealed two subunits of 97 and 74 kDa. Purified vitellin was used to raise polyclonal antisera, with which an enzyme-linked immunosorbent assay (ELISA) was developed. The ELISA was sensitive and could effectively detect vitellin in the range of 7.8-500 ng. Furthermore, vitellin levels in various developmental stages of oogenesis were measured with the ELISA assay. The results indicated that levels of vitellin increased significantly from 0.22 mg/ovary at Stage II to 360.31 mg/ovary at Stage IV.

KW - Decapoda

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KW - Enzyme-linked immunosorbent assay

KW - Eriocheir sinensis

KW - Gel filtration chromatography

KW - Ovary

KW - Purification

KW - Vitellin

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Purification of vitellin from the ovary of Chinese mitten-handed crab (Eriocheir sinensis) and development of an antivitellin ELISA

Abstract

Keywords

ASJC Scopus subject areas

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