Purification of vitellin from the ovary of Chinese mitten-handed crab (Eriocheir sinensis) and development of an antivitellin ELISA

Liqiao Chen, Hongbo Jiang, Zhongliang Zhou, Kang Li, Kai Li, Glenn Y. Deng, Zhanjiang Liu

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Vitellin was purified from ovaries of mature female Chinese mitten-handed crab (Eriocheir sinensis) using gel filtration chromatography. Analysis by native PAGE showed the vitellin had a native molecular mass of 520 kDa, while denaturing SDS-PAGE revealed two subunits of 97 and 74 kDa. Purified vitellin was used to raise polyclonal antisera, with which an enzyme-linked immunosorbent assay (ELISA) was developed. The ELISA was sensitive and could effectively detect vitellin in the range of 7.8-500 ng. Furthermore, vitellin levels in various developmental stages of oogenesis were measured with the ELISA assay. The results indicated that levels of vitellin increased significantly from 0.22 mg/ovary at Stage II to 360.31 mg/ovary at Stage IV.

Original languageEnglish (US)
Pages (from-to)305-311
Number of pages7
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume138
Issue number3
DOIs
StatePublished - Jul 2004
Externally publishedYes

Keywords

  • Decapoda
  • Developmental stages
  • Enzyme-linked immunosorbent assay
  • Eriocheir sinensis
  • Gel filtration chromatography
  • Ovary
  • Purification
  • Vitellin

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology

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