Purification and characterization of two new c-type cytochromes involved in Fe2+ oxidation from Thiobacillus ferrooxidans

Maria B. Valkova-Valchanova; Samuel H.P. Chan

doi:10.1111/j.1574-6968.1994.tb07076.x

Purification and characterization of two new c-type cytochromes involved in Fe²⁺ oxidation from Thiobacillus ferrooxidans

Maria B. Valkova-Valchanova, Samuel H.P. Chan

Department of Biology

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Two new c-type cytochromes have been purified from cell membranes of the acidophilic Thiobacillus ferrooxidans. In contrast to a soluble cytochrome c with molecular mass of 14 kDa reported earlier, a membrane-bound cytochrome c with a mass of 21 kDa was solubilized with octylthioglucoside and purified to homogeneity. In addition, a high molecular mass c-type cytochrome (68 kDa) was also solubilized and purified using Triton X-100 as a detergent. Both acid-stable species are partially released during osmotic shock and chloroform treatment of the bacteria; they are integral components in the respiratory chain donating electrons to the terminal cytochrome oxidase.

Original language	English (US)
Pages (from-to)	61-69
Number of pages	9
Journal	FEMS Microbiology Letters
Volume	121
Issue number	1
DOIs	https://doi.org/10.1111/j.1574-6968.1994.tb07076.x
State	Published - Aug 1 1994

Keywords

Cytochrome c
Cytochrome oxidase
Thiobacillus ferrooxidans

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

Access to Document

10.1111/j.1574-6968.1994.tb07076.x

Cite this

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title = "Purification and characterization of two new c-type cytochromes involved in Fe2+ oxidation from Thiobacillus ferrooxidans",

abstract = "Two new c-type cytochromes have been purified from cell membranes of the acidophilic Thiobacillus ferrooxidans. In contrast to a soluble cytochrome c with molecular mass of 14 kDa reported earlier, a membrane-bound cytochrome c with a mass of 21 kDa was solubilized with octylthioglucoside and purified to homogeneity. In addition, a high molecular mass c-type cytochrome (68 kDa) was also solubilized and purified using Triton X-100 as a detergent. Both acid-stable species are partially released during osmotic shock and chloroform treatment of the bacteria; they are integral components in the respiratory chain donating electrons to the terminal cytochrome oxidase.",

keywords = "Cytochrome c, Cytochrome oxidase, Thiobacillus ferrooxidans",

author = "Valkova-Valchanova, {Maria B.} and Chan, {Samuel H.P.}",

note = "Funding Information: We would like to thank Dr. R. Slepeckyf or careful readingo f this paper. This study is supported in part by a grant-in-aidfr om the American Heart Association.",

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