Purification and characterization of two new c-type cytochromes involved in Fe2+ oxidation from Thiobacillus ferrooxidans

Maria B. Valkova-Valchanova, Samuel H.P. Chan

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


Two new c-type cytochromes have been purified from cell membranes of the acidophilic Thiobacillus ferrooxidans. In contrast to a soluble cytochrome c with molecular mass of 14 kDa reported earlier, a membrane-bound cytochrome c with a mass of 21 kDa was solubilized with octylthioglucoside and purified to homogeneity. In addition, a high molecular mass c-type cytochrome (68 kDa) was also solubilized and purified using Triton X-100 as a detergent. Both acid-stable species are partially released during osmotic shock and chloroform treatment of the bacteria; they are integral components in the respiratory chain donating electrons to the terminal cytochrome oxidase.

Original languageEnglish (US)
Pages (from-to)61-69
Number of pages9
JournalFEMS Microbiology Letters
Issue number1
StatePublished - Aug 1 1994


  • Cytochrome c
  • Cytochrome oxidase
  • Thiobacillus ferrooxidans

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics


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