@inbook{120b808ebf8b4d2495ce831721e45158,
title = "Purification and Biophysical Analysis of Microtubule-Severing Enzymes In Vitro",
abstract = "Microtubule-severing enzymes are a novel class of microtubule regulators. They are enzymes from the ATPases associated with various cellular activities family (AAA. +) that utilize ATP to cut microtubules into smaller filaments. Discovered over 20 years ago, there are still many open questions about severing enzymes. Both cellular and biochemical studies need to be pursued to fully understand how these enzymes function mechanistically in the cell. Here, we present methods to express, purify, and test the biophysical nature of these proteins in vitro to begin to address the biochemical and biophysical mechanisms of this important and novel group of microtubule destabilizers.",
keywords = "Fidgetin, Katanin, Microtubule destabilizer, Severing enzyme, Spastin",
author = "Diaz-Valencia, {Juan Daniel} and Megan Bailey and Ross, {Jennifer L.}",
note = "Funding Information: We would like to thank David Sharp, Dong “Ray” Zhang, and Suranjana Mukherjee for their collaboration, advise, and initial preparation of the bacmid and virus for the Drosophila GFP-katanin. This work was supported by March of Dimes Basil O{\textquoteright}Connor Starter Grant #5-FY09-46 and NSF CMMI-0928540 to J. L. R. J. L. R. was also supported by NSF MRI Grant DBI-0923318, and a Cottrell Scholars Award from Research Corporation for Science Advancement #20031. M. B. and J. D. D.-V. were supported by NSF Grant DMR-1207783.",
year = "2013",
doi = "10.1016/B978-0-12-407757-7.00013-X",
language = "English (US)",
series = "Methods in Cell Biology",
publisher = "Academic Press Inc.",
pages = "191--213",
booktitle = "Methods in Cell Biology",
address = "United States",
}