Purification and Biophysical Analysis of Microtubule-Severing Enzymes In Vitro

Juan Daniel Diaz-Valencia, Megan Bailey, Jennifer L. Ross

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Scopus citations

Abstract

Microtubule-severing enzymes are a novel class of microtubule regulators. They are enzymes from the ATPases associated with various cellular activities family (AAA. +) that utilize ATP to cut microtubules into smaller filaments. Discovered over 20 years ago, there are still many open questions about severing enzymes. Both cellular and biochemical studies need to be pursued to fully understand how these enzymes function mechanistically in the cell. Here, we present methods to express, purify, and test the biophysical nature of these proteins in vitro to begin to address the biochemical and biophysical mechanisms of this important and novel group of microtubule destabilizers.

Original languageEnglish (US)
Title of host publicationMethods in Cell Biology
PublisherAcademic Press Inc.
Pages191-213
Number of pages23
DOIs
StatePublished - 2013
Externally publishedYes

Publication series

NameMethods in Cell Biology
Volume115
ISSN (Print)0091-679X

Keywords

  • Fidgetin
  • Katanin
  • Microtubule destabilizer
  • Severing enzyme
  • Spastin

ASJC Scopus subject areas

  • Cell Biology

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  • Cite this

    Diaz-Valencia, J. D., Bailey, M., & Ross, J. L. (2013). Purification and Biophysical Analysis of Microtubule-Severing Enzymes In Vitro. In Methods in Cell Biology (pp. 191-213). (Methods in Cell Biology; Vol. 115). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-407757-7.00013-X