TY - JOUR
T1 - Proteins, transcripts, and genetic architecture of seminal fluid and sperm in the mosquito aedes aegypti
AU - Degner, Ethan C.
AU - Ahmed-Braimah, Yasir H.
AU - Borziak, Kirill
AU - Wolfner, Mariana F.
AU - Harrington, Laura C.
AU - Dorus, Steve
N1 - Funding Information:
This study was supported by NIH/NIAID grant R01AI095491 to MFW and LCH, NIH/NICHD grant R21HD088910 to S.D. and MFW, a Cornell Graduate School fellowship to ECD, and a Cornell Entomology Department Griswold grant to ECD and LCH. YHAB was supported by NIH/NICHD grant R01HD059060 to MFW and Andrew G. Clark. RNA-seq data and mass spectrometry data were made possible by NIH grants 1S10OD010693-01 and 1S10OD017992-01, respectively. Proteomics computing was supported by NSF grant OAC-1541396/ACI-1541396 to Eric Sedore of the Syracuse University Information Technology Services. Mosquito images in Graphical Abstract used with permission, © 2016 David Felix Duneau.
Funding Information:
* This study was supported by NIH/NIAID grant R01AI095491 to MFW and LCH, NIH/NICHD grant R21HD088910 to S.D. and MFW, a Cornell Graduate School fellowship to ECD, and a Cornell Entomology Department Griswold grant to ECD and LCH. YHAB was supported by NIH/NICHD grant R01HD059060 to MFW and Andrew G. Clark. RNA-seq data and mass spectrometry data were made possible by NIH grants 1S10OD010693-01 and 1S10OD017992-01, respectively. Proteomics computing was supported by NSF grant OAC-1541396/ACI-1541396 to Eric Sedore of the Syracuse University Information Technology Services. Mosquito images in Graphical Abstract used with permission, © 2016 David Felix Duneau. □S This article contains supplemental material. ‖ To whom correspondence may be addressed. E-mail: mfw5@ cornell.edu. ‡‡ To whom correspondence may be addressed. E-mail: lch27@ cornell.edu. §§ To whom correspondence may be addressed. E-mail: sdorus@ syr.edu. ** Authors contributed equally to this work.
Publisher Copyright:
© 2019 Degner et al. Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.
PY - 2019/3
Y1 - 2019/3
N2 - The yellow fever mosquito, Aedes aegypti, transmits several viruses causative of serious diseases, including dengue, Zika, and chikungunya. Some proposed efforts to control this vector involve manipulating reproduction to suppress wild populations or to replace them with disease-resistant mosquitoes. The design of such strategies requires an intimate knowledge of reproductive processes, yet our basic understanding of reproductive genetics in this vector remains largely incomplete. To accelerate future investigations, we have comprehensively catalogued sperm and seminal fluid proteins (SFPs) transferred to females in the ejaculate using tandem mass spectrometry. By excluding female-derived proteins using an isotopic labeling approach, we identified 870 sperm proteins and 280 SFPs. Functional composition analysis revealed parallels with known aspects of sperm biology and SFP function in other insects. To corroborate our proteome characterization, we also generated transcriptomes for testes and the male accessory glands-the primary contributors to Ae. aegypti sperm and seminal fluid, respectively. Differential gene expression of accessory glands from virgin and mated males suggests that transcripts encoding proteins involved in protein translation are upregulated post-mating. Several SFP transcripts were also modulated after mating, but >90% remained unchanged. Finally, a significant enrichment of SFPs was observed on chromosome 1, which harbors the male sex determining locus in this species. Our study provides a comprehensive proteomic and transcriptomic characterization of ejaculate production and composition and thus provides a foundation for future investigations of Ae. aegypti reproductive biology, from functional analysis of individual proteins to broader examination of reproductive processes.
AB - The yellow fever mosquito, Aedes aegypti, transmits several viruses causative of serious diseases, including dengue, Zika, and chikungunya. Some proposed efforts to control this vector involve manipulating reproduction to suppress wild populations or to replace them with disease-resistant mosquitoes. The design of such strategies requires an intimate knowledge of reproductive processes, yet our basic understanding of reproductive genetics in this vector remains largely incomplete. To accelerate future investigations, we have comprehensively catalogued sperm and seminal fluid proteins (SFPs) transferred to females in the ejaculate using tandem mass spectrometry. By excluding female-derived proteins using an isotopic labeling approach, we identified 870 sperm proteins and 280 SFPs. Functional composition analysis revealed parallels with known aspects of sperm biology and SFP function in other insects. To corroborate our proteome characterization, we also generated transcriptomes for testes and the male accessory glands-the primary contributors to Ae. aegypti sperm and seminal fluid, respectively. Differential gene expression of accessory glands from virgin and mated males suggests that transcripts encoding proteins involved in protein translation are upregulated post-mating. Several SFP transcripts were also modulated after mating, but >90% remained unchanged. Finally, a significant enrichment of SFPs was observed on chromosome 1, which harbors the male sex determining locus in this species. Our study provides a comprehensive proteomic and transcriptomic characterization of ejaculate production and composition and thus provides a foundation for future investigations of Ae. aegypti reproductive biology, from functional analysis of individual proteins to broader examination of reproductive processes.
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U2 - 10.1074/mcp.RA118.001067
DO - 10.1074/mcp.RA118.001067
M3 - Article
C2 - 30552291
AN - SCOPUS:85063279537
SN - 1535-9476
VL - 18
SP - S6-S22
JO - Molecular and Cellular Proteomics
JF - Molecular and Cellular Proteomics
ER -