Protein sensing with engineered protein nanopores

Mohammad M. Mohammad, Liviu Movileanu

Research output: ResearchChapter

  • 6 Citations

Abstract

The use of nanopores is a powerful new frontier in single-molecule sciences. Nanopores have been used effectively in exploring various biophysical features of small polypeptides and proteins, such as their folding state and structure, ligand interactions, and enzymatic activity. In particular, the α-hemolysin (αHL) protein pore has been used extensively for the detection, characterization, and analysis of polypeptides because this protein nanopore is highly robust, versatile, and tractable under various experimental conditions. Inspired by the mechanisms of protein translocation across the outer membrane translocases of mitochondria, we have shown the ability to use nanopore-probe techniques in controlling a single protein using engineered αHL pores. Here, we provide a detailed protocol for the preparation of αHL protein nanopores. Moreover, we demonstrate that placing attractive electrostatic traps is instrumental in tackling single-molecule stochastic sensing of folded proteins.

LanguageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages21-37
Number of pages17
Volume870
DOIs
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
Volume870
ISSN (Print)10643745

Fingerprint

Nanopores
Proteins
Hemolysin Proteins
Peptides
Protein Transport
Static Electricity
Mitochondria
Ligands
Membranes

Keywords

  • α-Hemolysin
  • Biotechnology and biosensors
  • Nanopore
  • Protein engineering
  • Single-channel electrical recordings

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Mohammad, M. M., & Movileanu, L. (2012). Protein sensing with engineered protein nanopores. In Methods in Molecular Biology (Vol. 870, pp. 21-37). (Methods in Molecular Biology; Vol. 870). DOI: 10.1007/978-1-61779-773-6_2

Protein sensing with engineered protein nanopores. / Mohammad, Mohammad M.; Movileanu, Liviu.

Methods in Molecular Biology. Vol. 870 2012. p. 21-37 (Methods in Molecular Biology; Vol. 870).

Research output: ResearchChapter

Mohammad, MM & Movileanu, L 2012, Protein sensing with engineered protein nanopores. in Methods in Molecular Biology. vol. 870, Methods in Molecular Biology, vol. 870, pp. 21-37. DOI: 10.1007/978-1-61779-773-6_2
Mohammad MM, Movileanu L. Protein sensing with engineered protein nanopores. In Methods in Molecular Biology. Vol. 870. 2012. p. 21-37. (Methods in Molecular Biology). Available from, DOI: 10.1007/978-1-61779-773-6_2
Mohammad, Mohammad M. ; Movileanu, Liviu. / Protein sensing with engineered protein nanopores. Methods in Molecular Biology. Vol. 870 2012. pp. 21-37 (Methods in Molecular Biology).
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