Protein posttranslational modification

James L. Hougland, Joseph Darling, Susan Flynn

Research output: Chapter in Book/Entry/PoemChapter

15 Scopus citations


Proteins play central and essential roles in the vast majority of biological processes. Protein function is transduced through molecular interactions involving amino acid side chains. This chapter focuses on the protein modifications that occur due to reactive species generated during oxidative stress. It presents an overview of the modifications that can occur at each amino acid due to oxidative stress and a survey of the methods currently in use to detect oxidative stress‐related posttranslational modifications (PTMs). Among the array of methods that have been used to identify oxidative PTMs, mass spectrometry and chemoselective modifications have proven to be the most robust and specific tools for identifying these modifications within the proteome. A section provides an overview of the current state of both techniques, with more detailed descriptions. The chapter concludes with a discussion on the role of PTMs in cellular redox signaling.

Original languageEnglish (US)
Title of host publicationMolecular Basis of Oxidative Stress
Subtitle of host publicationChemistry, Mechanisms, and Disease Pathogenesis
Number of pages22
ISBN (Electronic)9780470572184
ISBN (Print)9781118355886
StatePublished - Jan 1 2013


  • Amino acid modification
  • Cellular redox signaling
  • Posttranslational modifications (PTMs)
  • Protein posttranslational modification

ASJC Scopus subject areas

  • General Chemistry
  • General Chemical Engineering
  • General Pharmacology, Toxicology and Pharmaceutics
  • General Biochemistry, Genetics and Molecular Biology


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