Progressive Myofibril Reorganization of Human Cardiomyocytes on a Dynamic Nanotopographic Substrate

Shiyang Sun, Huaiyu Shi, Sarah Moore, Chenyan Wang, Ariel Ash-Shakoor, Patrick T. Mather, James H. Henderson, Zhen Ma

Research output: Contribution to journalArticle

Abstract

Cardiomyocyte (CM) alignment with striated myofibril organization is developed during early cardiac organogenesis. Previous work has successfully achieved in vitro CM alignment using a variety of biomaterial scaffolds and substrates with static topographic features. However, the cellular processes that occur during the response of CMs to dynamic surface topographic changes, which may provide a model of in vivo developmental progress of CM alignment within embryonic myocardium, remains poorly understood. To gain insights into these cellular processes involved in the response of CMs to dynamic topographic changes, we developed a dynamic topographic substrate that employs a shape memory polymer coated with polyelectrolyte multilayers to produce a flat-to-wrinkle surface transition when triggered by a change in incubation temperature. Using this system, we investigated cellular morphological alignment and intracellular myofibril reorganization in response to the dynamic wrinkle formation. Hence, we identified the progressive cellular processes of human-induced pluripotent stem cell-CMs in a time-dependent manner, which could provide a foundation for a mechanistic model of cardiac myofibril reorganization in response to extracellular microenvironment changes.

Original languageEnglish (US)
Pages (from-to)21450-21462
Number of pages13
JournalACS Applied Materials and Interfaces
Volume12
Issue number19
DOIs
StatePublished - May 13 2020

Keywords

  • cardiac mechanobiology
  • human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)
  • nanotopography
  • shape memory polymer (SMP)
  • stimuli-responsive biomaterials

ASJC Scopus subject areas

  • Materials Science(all)

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