Probing Bioluminescence Resonance Energy Transfer in Quantum Rod-Luciferase Nanoconjugates

Rabeka Alam, Liliana M. Karam, Tennyson L. Doane, Kaitlin Coopersmith, Danielle M. Fontaine, Bruce R. Branchini, Mathew M Maye

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We describe the necessary design criteria to create highly efficient energy transfer conjugates containing luciferase enzymes derived from Photinus pyralis (Ppy) and semiconductor quantum rods (QRs) with rod-in-rod (r/r) microstructure. By fine-tuning the synthetic conditions, CdSe/CdS r/r-QRs were prepared with two different emission colors and three different aspect ratios (l/w) each. These were hybridized with blue, green, and red emitting Ppy, leading to a number of new BRET nanoconjugates. Measurements of the emission BRET ratio (BR) indicate that the resulting energy transfer is highly dependent on QR energy accepting properties, which include absorption, quantum yield, and optical anisotropy, as well as its morphological and topological properties, such as aspect ratio and defect concentration. The highest BR was found using r/r-QRs with lower l/w that were conjugated with red Ppy, which may be activating one of the anisotropic CdSe core energy levels. The role QR surface defects play on Ppy binding, and energy transfer was studied by growth of gold nanoparticles at the defects, which indicated that each QR set has different sites. The Ppy binding at those sites is suggested by the observed BRET red-shift as a function of Ppy-to-QR loading (L), where the lowest L results in highest efficiency and furthest shift.

Original languageEnglish (US)
Pages (from-to)1969-1977
Number of pages9
JournalACS Nano
Volume10
Issue number2
DOIs
StatePublished - Feb 23 2016

Fingerprint

Nanoconjugates
Bioluminescence
bioluminescence
Luciferases
Energy transfer
rods
energy transfer
Aspect ratio
Optical anisotropy
Defects
Surface defects
Quantum yield
Gold
Electron energy levels
Enzymes
Tuning
Semiconductor materials
Nanoparticles
Color
Microstructure

Keywords

  • bioluminescence
  • biotic/abiotic
  • BRET
  • energy transfer
  • FRET
  • luciferase
  • nanoconjugate
  • polarization
  • quantum rods
  • rod-in-rod

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

Cite this

Alam, R., Karam, L. M., Doane, T. L., Coopersmith, K., Fontaine, D. M., Branchini, B. R., & Maye, M. M. (2016). Probing Bioluminescence Resonance Energy Transfer in Quantum Rod-Luciferase Nanoconjugates. ACS Nano, 10(2), 1969-1977. https://doi.org/10.1021/acsnano.5b05966

Probing Bioluminescence Resonance Energy Transfer in Quantum Rod-Luciferase Nanoconjugates. / Alam, Rabeka; Karam, Liliana M.; Doane, Tennyson L.; Coopersmith, Kaitlin; Fontaine, Danielle M.; Branchini, Bruce R.; Maye, Mathew M.

In: ACS Nano, Vol. 10, No. 2, 23.02.2016, p. 1969-1977.

Research output: Contribution to journalArticle

Alam, R, Karam, LM, Doane, TL, Coopersmith, K, Fontaine, DM, Branchini, BR & Maye, MM 2016, 'Probing Bioluminescence Resonance Energy Transfer in Quantum Rod-Luciferase Nanoconjugates', ACS Nano, vol. 10, no. 2, pp. 1969-1977. https://doi.org/10.1021/acsnano.5b05966
Alam R, Karam LM, Doane TL, Coopersmith K, Fontaine DM, Branchini BR et al. Probing Bioluminescence Resonance Energy Transfer in Quantum Rod-Luciferase Nanoconjugates. ACS Nano. 2016 Feb 23;10(2):1969-1977. https://doi.org/10.1021/acsnano.5b05966
Alam, Rabeka ; Karam, Liliana M. ; Doane, Tennyson L. ; Coopersmith, Kaitlin ; Fontaine, Danielle M. ; Branchini, Bruce R. ; Maye, Mathew M. / Probing Bioluminescence Resonance Energy Transfer in Quantum Rod-Luciferase Nanoconjugates. In: ACS Nano. 2016 ; Vol. 10, No. 2. pp. 1969-1977.
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