Preparation of Hydrazino-Modified Proteins and Their Use for the Synthesis of 99mTc-Protein Conjugates

David A. Schwartz; Michael J. Abrams; Marguerite M. Hauser; Forrest E. Gaul; Scott K. Larsen; Donald Rauh; Jon A. Zubieta

doi:10.1021/bc00011a007

Preparation of Hydrazino-Modified Proteins and Their Use for the Synthesis of ^99mTc-Protein Conjugates

David A. Schwartz, Michael J. Abrams, Marguerite M. Hauser, Forrest E. Gaul, Scott K. Larsen, Donald Rauh, Jon A. Zubieta

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

114 Scopus citations

Abstract

The syntheses and protein linking properties of succinimidyl 4-hydrazinobenzoate hydrochloride (SHBH) and succinimidyl 6-hydrazinonicotinate hydrochloride (SHNH), two new heterobifunctional linkers which lead to hydrazino-modified proteins, are described. SHBH-modified proteins are unstable due to the presence of the phenylhydrazine moiety. This problem was overcome by synthesizing the hydrazinopyridine analogue SHNH, and the conjugates derived from this linker are stable. Tc(V) oxo precursors readily add to hydrazinopyridine-modified proteins to yield the desired * Tc-radiolabeled protein. ^99mTc-hydrazinopyridine-polyclonal IgG conjugates are useful agents for the imaging of focal sites of infection.

Original language	English (US)
Pages (from-to)	333-336
Number of pages	4
Journal	Bioconjugate Chemistry
Volume	2
Issue number	5
DOIs	https://doi.org/10.1021/bc00011a007
State	Published - Sep 1 1991

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biomedical Engineering
Pharmacology
Pharmaceutical Science
Organic Chemistry

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title = "Preparation of Hydrazino-Modified Proteins and Their Use for the Synthesis of 99mTc-Protein Conjugates",

abstract = "The syntheses and protein linking properties of succinimidyl 4-hydrazinobenzoate hydrochloride (SHBH) and succinimidyl 6-hydrazinonicotinate hydrochloride (SHNH), two new heterobifunctional linkers which lead to hydrazino-modified proteins, are described. SHBH-modified proteins are unstable due to the presence of the phenylhydrazine moiety. This problem was overcome by synthesizing the hydrazinopyridine analogue SHNH, and the conjugates derived from this linker are stable. Tc(V) oxo precursors readily add to hydrazinopyridine-modified proteins to yield the desired * Tc-radiolabeled protein. 99mTc-hydrazinopyridine-polyclonal IgG conjugates are useful agents for the imaging of focal sites of infection.",

author = "Schwartz, {David A.} and Abrams, {Michael J.} and Hauser, {Marguerite M.} and Gaul, {Forrest E.} and Larsen, {Scott K.} and Donald Rauh and Zubieta, {Jon A.}",

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T1 - Preparation of Hydrazino-Modified Proteins and Their Use for the Synthesis of 99mTc-Protein Conjugates

AU - Schwartz, David A.

AU - Abrams, Michael J.

AU - Hauser, Marguerite M.

AU - Gaul, Forrest E.

AU - Larsen, Scott K.

AU - Rauh, Donald

AU - Zubieta, Jon A.

PY - 1991/9/1

Y1 - 1991/9/1

N2 - The syntheses and protein linking properties of succinimidyl 4-hydrazinobenzoate hydrochloride (SHBH) and succinimidyl 6-hydrazinonicotinate hydrochloride (SHNH), two new heterobifunctional linkers which lead to hydrazino-modified proteins, are described. SHBH-modified proteins are unstable due to the presence of the phenylhydrazine moiety. This problem was overcome by synthesizing the hydrazinopyridine analogue SHNH, and the conjugates derived from this linker are stable. Tc(V) oxo precursors readily add to hydrazinopyridine-modified proteins to yield the desired * Tc-radiolabeled protein. 99mTc-hydrazinopyridine-polyclonal IgG conjugates are useful agents for the imaging of focal sites of infection.

AB - The syntheses and protein linking properties of succinimidyl 4-hydrazinobenzoate hydrochloride (SHBH) and succinimidyl 6-hydrazinonicotinate hydrochloride (SHNH), two new heterobifunctional linkers which lead to hydrazino-modified proteins, are described. SHBH-modified proteins are unstable due to the presence of the phenylhydrazine moiety. This problem was overcome by synthesizing the hydrazinopyridine analogue SHNH, and the conjugates derived from this linker are stable. Tc(V) oxo precursors readily add to hydrazinopyridine-modified proteins to yield the desired * Tc-radiolabeled protein. 99mTc-hydrazinopyridine-polyclonal IgG conjugates are useful agents for the imaging of focal sites of infection.

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Preparation of Hydrazino-Modified Proteins and Their Use for the Synthesis of ^99mTc-Protein Conjugates

Abstract

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