Preparation of hydrazino-modified proteins and their use for the synthesis of 99mTc-protein conjugates

David A. Schwartz, Michael J. Abrams, Marguerite M. Hauser, Forrest E. Gaul, Scott K. Larsen, Donald Rauh, Jon A Zubieta

Research output: Contribution to journalArticle

111 Scopus citations

Abstract

The syntheses and protein linking properties of succinimidyl 4-hydrazinobenzoate hydrochloride (SHBH) and succinimidyl 6-hydrazinonicotinate hydrochloride (SHNH), two new heterobifunctional linkers which lead to hydrazino-modified proteins, are described. SHBH-modified proteins are unstable due to the presence of the phenylhydrazine moiety. This problem was overcome by synthesizing the hydrazinopyridine analogue SHNH, and the conjugates derived from this linker are stable. Tc(V) oxo precursors readily add to hydrazinopyridine-modified proteins to yield the desired 99mTc-radiolabeled protein. 99mTc-hydrazinopyridine-polyclonal IgG conjugates are useful agents for the imaging of focal sites of infection.

Original languageEnglish (US)
Pages (from-to)333-336
Number of pages4
JournalBioconjugate Chemistry
Volume2
Issue number5
StatePublished - 1991

    Fingerprint

ASJC Scopus subject areas

  • Chemistry(all)
  • Organic Chemistry
  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Schwartz, D. A., Abrams, M. J., Hauser, M. M., Gaul, F. E., Larsen, S. K., Rauh, D., & Zubieta, J. A. (1991). Preparation of hydrazino-modified proteins and their use for the synthesis of 99mTc-protein conjugates. Bioconjugate Chemistry, 2(5), 333-336.