Preparation of Hydrazino-Modified Proteins and Their Use for the Synthesis of 99mTc-Protein Conjugates

David A. Schwartz, Michael J. Abrams, Marguerite M. Hauser, Forrest E. Gaul, Scott K. Larsen, Donald Rauh, Jon A. Zubieta

Research output: Contribution to journalArticle

112 Scopus citations

Abstract

The syntheses and protein linking properties of succinimidyl 4-hydrazinobenzoate hydrochloride (SHBH) and succinimidyl 6-hydrazinonicotinate hydrochloride (SHNH), two new heterobifunctional linkers which lead to hydrazino-modified proteins, are described. SHBH-modified proteins are unstable due to the presence of the phenylhydrazine moiety. This problem was overcome by synthesizing the hydrazinopyridine analogue SHNH, and the conjugates derived from this linker are stable. Tc(V) oxo precursors readily add to hydrazinopyridine-modified proteins to yield the desired * Tc-radiolabeled protein. 99mTc-hydrazinopyridine-polyclonal IgG conjugates are useful agents for the imaging of focal sites of infection.

Original languageEnglish (US)
Pages (from-to)333-336
Number of pages4
JournalBioconjugate Chemistry
Volume2
Issue number5
DOIs
StatePublished - Sep 1 1991

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Preparation of Hydrazino-Modified Proteins and Their Use for the Synthesis of <sup>99m</sup>Tc-Protein Conjugates'. Together they form a unique fingerprint.

  • Cite this