Preparation of antibody-conjugated gold nanoparticles

Anthony J. Di Pasqua; Richard E. Mishler; Yan Li Ship; James C. Dabrowiak; Tewodros Asefa

doi:10.1016/j.matlet.2009.05.070

Preparation of antibody-conjugated gold nanoparticles

Anthony J. Di Pasqua, Richard E. Mishler, Yan Li Ship, James C. Dabrowiak, Tewodros Asefa

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

71 Scopus citations

Abstract

We here describe a method for the simple synthesis of gold nanoparticles (~ 10 nm in diameter) conjugated with antibody to E. coli O157:H7. Gold nanoparticles with pendant carboxylic acid and alcohol functional groups were synthesized and characterized using transmission electron microscopy (TEM) and infrared spectroscopy. These nanoparticles were then reacted with anti-E. coli O157:H7, using EDC coupling chemistry, and the product was characterized with X-ray photoelectron spectroscopy. Furthermore, binding of the antibody-gold conjugates to E. coli O157:H7 was demonstrated using transmission electron microscopy.

Original language	English (US)
Pages (from-to)	1876-1879
Number of pages	4
Journal	Materials Letters
Volume	63
Issue number	21
DOIs	https://doi.org/10.1016/j.matlet.2009.05.070
State	Published - Aug 31 2009

Keywords

Antibody
E. coli
Gold nanoparticles
Pathogen

ASJC Scopus subject areas

General Materials Science
Condensed Matter Physics
Mechanics of Materials
Mechanical Engineering

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10.1016/j.matlet.2009.05.070

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@article{a6b2926d75a84b6a97f8846465c14937,

title = "Preparation of antibody-conjugated gold nanoparticles",

abstract = "We here describe a method for the simple synthesis of gold nanoparticles (~ 10 nm in diameter) conjugated with antibody to E. coli O157:H7. Gold nanoparticles with pendant carboxylic acid and alcohol functional groups were synthesized and characterized using transmission electron microscopy (TEM) and infrared spectroscopy. These nanoparticles were then reacted with anti-E. coli O157:H7, using EDC coupling chemistry, and the product was characterized with X-ray photoelectron spectroscopy. Furthermore, binding of the antibody-gold conjugates to E. coli O157:H7 was demonstrated using transmission electron microscopy.",

keywords = "Antibody, E. coli, Gold nanoparticles, Pathogen",

author = "{Di Pasqua}, {Anthony J.} and Mishler, {Richard E.} and Ship, {Yan Li} and Dabrowiak, {James C.} and Tewodros Asefa",

note = "Funding Information: We gratefully acknowledge support for this work by Syracuse Center of Excellence CARTI project award, which is supported by a grant from U.S. Environmental Protection Agency [Award No: X-83232501-0]. This work has not been subjected to the Agency's required peer and policy review and therefore does not necessarily reflect the views of the agency and no official endorsement shall be inferred. We thank Mr. Anthony Terrinoni of Antek Inc. in Syracuse, NY for supplying us with E. coli samples.",

year = "2009",

month = aug,

day = "31",

doi = "10.1016/j.matlet.2009.05.070",

language = "English (US)",

volume = "63",

pages = "1876--1879",

journal = "Materials Letters",

issn = "0167-577X",

publisher = "Elsevier",

number = "21",

}

TY - JOUR

T1 - Preparation of antibody-conjugated gold nanoparticles

AU - Di Pasqua, Anthony J.

AU - Mishler, Richard E.

AU - Ship, Yan Li

AU - Dabrowiak, James C.

AU - Asefa, Tewodros

N1 - Funding Information: We gratefully acknowledge support for this work by Syracuse Center of Excellence CARTI project award, which is supported by a grant from U.S. Environmental Protection Agency [Award No: X-83232501-0]. This work has not been subjected to the Agency's required peer and policy review and therefore does not necessarily reflect the views of the agency and no official endorsement shall be inferred. We thank Mr. Anthony Terrinoni of Antek Inc. in Syracuse, NY for supplying us with E. coli samples.

PY - 2009/8/31

Y1 - 2009/8/31

N2 - We here describe a method for the simple synthesis of gold nanoparticles (~ 10 nm in diameter) conjugated with antibody to E. coli O157:H7. Gold nanoparticles with pendant carboxylic acid and alcohol functional groups were synthesized and characterized using transmission electron microscopy (TEM) and infrared spectroscopy. These nanoparticles were then reacted with anti-E. coli O157:H7, using EDC coupling chemistry, and the product was characterized with X-ray photoelectron spectroscopy. Furthermore, binding of the antibody-gold conjugates to E. coli O157:H7 was demonstrated using transmission electron microscopy.

AB - We here describe a method for the simple synthesis of gold nanoparticles (~ 10 nm in diameter) conjugated with antibody to E. coli O157:H7. Gold nanoparticles with pendant carboxylic acid and alcohol functional groups were synthesized and characterized using transmission electron microscopy (TEM) and infrared spectroscopy. These nanoparticles were then reacted with anti-E. coli O157:H7, using EDC coupling chemistry, and the product was characterized with X-ray photoelectron spectroscopy. Furthermore, binding of the antibody-gold conjugates to E. coli O157:H7 was demonstrated using transmission electron microscopy.

KW - Antibody

KW - E. coli

KW - Gold nanoparticles

KW - Pathogen

UR - http://www.scopus.com/inward/record.url?scp=67649367517&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67649367517&partnerID=8YFLogxK

U2 - 10.1016/j.matlet.2009.05.070

DO - 10.1016/j.matlet.2009.05.070

M3 - Article

AN - SCOPUS:67649367517

SN - 0167-577X

VL - 63

SP - 1876

EP - 1879

JO - Materials Letters

JF - Materials Letters

IS - 21

ER -

Preparation of antibody-conjugated gold nanoparticles

Abstract

Keywords

ASJC Scopus subject areas

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