Preparation and screening of catalytic amyloid assemblies

Zsofia Lengyel, Caroline M. Rufo, Ivan Korendovych

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

Aggregation of proteins into amyloids has long been recognized as one of the major contributors to disease and aging. Amyloids are known to catalyze their own formation but they have been considered the rock-bottom thermodynamic minimum of the protein fold without much functionality. We have recently demonstrated that aggregation of short peptides in the presence of metal ions gives rise to efficient catalytic activity. Here we present a detailed protocol for the synthesis and purification of these peptides and the preparation of amyloid-like fibrils. Then we describe an easy-to-perform, high-throughput assay to measure their hydrolytic activity.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages261-270
Number of pages10
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1777
ISSN (Print)1064-3745

Keywords

  • Amyloids
  • Catalysis
  • Hydrolysis
  • Peptides
  • Self-assembly

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Lengyel, Z., Rufo, C. M., & Korendovych, I. (2018). Preparation and screening of catalytic amyloid assemblies. In Methods in Molecular Biology (pp. 261-270). (Methods in Molecular Biology; Vol. 1777). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7811-3_16