TY - JOUR
T1 - Phototropism mutants of Phycomyces blakesleeanus isolated at low light intensity
AU - Alvarez, Maria Isabel
AU - Eslava, Arturo P.
AU - Lipson, Edward D.
N1 - Funding Information:
We thank Promod Pratap for assistance with plotting of the figures. This work was supported by grants from the National Science Foundation (DMB-8704602) and the National Institutes of Health (GM-29707). M.I.A. and A.P.E. wish to thank the Direction General de Investigation Cientifica y Tecnica of Spain for financial support during their sabbatical stay at Syracuse University.
PY - 1989/3
Y1 - 1989/3
N2 - Mutants of Phycomyces have played a major role in the analysis of phototropism and other responses. Fifteen new mutants of Phycomyces with abnormal phototropism (genotype mad) have been isolated on the basis of their inability to bend toward dim unilateral blue light (fluence rate 5 × 10-7 W m-2), a protocol different from those employed in previous mutant hunts. One mutant resulted from chemical mutagenesis with ICR-170, and the other 14 were induced with N-methyl-N′-nitro-N-nitro-soguanidine. Seven of the mutants are "night blind"; six have phototropic thresholds intermediate between those of wild type (10-9 W m-2) and madA strains (∼ 10-4 W m-2); and one has a threshold similar to that of night-blind madB and madC mutants. The other eight mutants are "stiff", with various reductions of tropic responsiveness. Two of them, when compared to previously isolated stiff mutants, show unusually weak responses to light, barriers, and gravity.
AB - Mutants of Phycomyces have played a major role in the analysis of phototropism and other responses. Fifteen new mutants of Phycomyces with abnormal phototropism (genotype mad) have been isolated on the basis of their inability to bend toward dim unilateral blue light (fluence rate 5 × 10-7 W m-2), a protocol different from those employed in previous mutant hunts. One mutant resulted from chemical mutagenesis with ICR-170, and the other 14 were induced with N-methyl-N′-nitro-N-nitro-soguanidine. Seven of the mutants are "night blind"; six have phototropic thresholds intermediate between those of wild type (10-9 W m-2) and madA strains (∼ 10-4 W m-2); and one has a threshold similar to that of night-blind madB and madC mutants. The other eight mutants are "stiff", with various reductions of tropic responsiveness. Two of them, when compared to previously isolated stiff mutants, show unusually weak responses to light, barriers, and gravity.
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U2 - 10.1016/0147-5975(89)90006-6
DO - 10.1016/0147-5975(89)90006-6
M3 - Article
AN - SCOPUS:38249023449
SN - 1087-1845
VL - 13
SP - 38
EP - 48
JO - Fungal Genetics and Biology
JF - Fungal Genetics and Biology
IS - 1
ER -