Phosphoinositide 3-kinase family in channel catfish and their regulated expression after bacterial infection

Zhaoxia Li, Jun Yao, Yangjie Xie, Xin Geng, Zhanjian "John" Liu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The phosphoinositide-3-kinase (PI3Ks) family of lipid kinases is widely conserved from yeast to mammals. In this work, we identified a total of 14 members of the PI3Ks from the channel catfish genome and transcriptome and conducted phylogenetic and syntenic analyses of these genes. The expression profiles after infection with Edwardsiella ictaluri and Flavobacterium columnare were examined to determine the involvement of PI3Ks in immune responses after bacterial infection in catfish. The results indicated that PI3Ks genes including all of the catalytic subunit and several regulatory subunits genes were widely regulated after bacterial infection. The expression patterns were quite different when challenged with different bacteria. The PI3Ks were up-regulated rapidly at the early stage after ESC infection, but their induced expression was much slower, at the middle stage after columnaris infection. RNA-Seq datasets indicated that PI3K genes may be expressed at different levels in different catfish differing in their resistance levels against columnaris. Future studies are required to confirm and validate these observations. Taken together, this study indicated that PI3K genes may be involved as a part of the defense responses of catfish after infections, and they could be one of the determinants for disease resistance.

Original languageEnglish (US)
Pages (from-to)364-373
Number of pages10
JournalFish and Shellfish Immunology
Volume49
DOIs
StatePublished - Feb 1 2016
Externally publishedYes

Fingerprint

phosphatidylinositol 3-kinase
Ictalurus punctatus
Phosphatidylinositols
bacterial infections
Phosphotransferases
Genes
gene
catfish
genes
infection
Flavobacterium columnare
Edwardsiella ictaluri
disease resistance
immune response
protein subunits
Mammals
yeast
family
RNA
transcriptome

Keywords

  • Fish
  • Gene expression
  • Genome
  • Immune response
  • Phosphoinositol kinase
  • PI3K

ASJC Scopus subject areas

  • Environmental Chemistry
  • Aquatic Science

Cite this

Phosphoinositide 3-kinase family in channel catfish and their regulated expression after bacterial infection. / Li, Zhaoxia; Yao, Jun; Xie, Yangjie; Geng, Xin; Liu, Zhanjian "John".

In: Fish and Shellfish Immunology, Vol. 49, 01.02.2016, p. 364-373.

Research output: Contribution to journalArticle

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