PCR generation of large amounts of purified DNA

Liliana Falzon, Chris Kirk, Jonathan B. Chaires, James C. Dabrowiak

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The preparation and purification of PCR generated DNA fragments suitable for footprinting and classical drug binding studies is described. One of the fragments, a 214-mer derived from pBR322 DNA exhibits a biphasic melting profile. This behavior appears to be due to a non-random distribution of base pairs within the fragment causing a region rich in AT base pairs to melt prior to a segment having a high concentration of GC base pairs. The usefulness of large amounts of PCR generated DNA for footprinting and optical binding studies involving drugs is also presented and discussed.

Original languageEnglish (US)
Pages (from-to)251-257
Number of pages7
JournalJournal of Biochemical and Biophysical Methods
Volume29
Issue number3-4
DOIs
StatePublished - Dec 1994

Keywords

  • DNA melting
  • Drug binding
  • PCR
  • Specificity

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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