Abstract
The preparation and purification of PCR generated DNA fragments suitable for footprinting and classical drug binding studies is described. One of the fragments, a 214-mer derived from pBR322 DNA exhibits a biphasic melting profile. This behavior appears to be due to a non-random distribution of base pairs within the fragment causing a region rich in AT base pairs to melt prior to a segment having a high concentration of GC base pairs. The usefulness of large amounts of PCR generated DNA for footprinting and optical binding studies involving drugs is also presented and discussed.
Original language | English (US) |
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Pages (from-to) | 251-257 |
Number of pages | 7 |
Journal | Journal of Biochemical and Biophysical Methods |
Volume | 29 |
Issue number | 3-4 |
DOIs | |
State | Published - Dec 1994 |
Keywords
- DNA melting
- Drug binding
- PCR
- Specificity
ASJC Scopus subject areas
- Biophysics
- Biochemistry