Pathogen recognition receptors in channel catfish: IV. Identification, phylogeny and expression analysis of peptidoglycan recognition proteins

Luyang Sun, Shikai Liu, Ruijia Wang, Chao Li, Jiaren Zhang, Zhanjiang Liu

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Peptidoglycan recognition proteins (PGRPs) can recognize bacterial cell wall (peptidoglycan) and activate innate immune system. In addition to its function as pathogen recognition receptors (PRRs), PGRPs are also involved in directly killing bacteria, and regulating multiple signaling pathways. Recently, we have reported catfish PRRs including nucleotide-binding domain, leucine-rich repeat containing receptors (NLRs), retinoic acid inducible gene I (RIG-I) like receptors (RLRs), and Toll-like receptors (TLRs). In this study, we identified and characterized the PGRP gene family in channel catfish which included two members, PGLYRP-5 and PGLYRP-6. Phylogenetic analysis, syntenic analysis and protein structural analysis were conducted to determine their identities and evolutionary relationships. In order to gain insight into the roles of PGRPs in catfish innate immune responses, quantitative real-time PCR was used to investigate the expression profiles in catfish healthy tissues and after bacterial infection. Both PGLYRP-5 and PGLYRP-6 were ubiquitously expressed in all 12 healthy tissues, and most highly expressed in gill and spleen, respectively. Distinct expression patterns were observed for PGRPs after infection with Edwardsiella ictaluri and Flavobacterium columnare, both Gram-negative bacteria. After infection with E. ictaluri, both PGLYRP-5 and PGLYRP-6 were significantly down-regulated at a certain time-point, while both genes were generally up-regulated in the gill after infection with F. columnare. Collectively, these findings suggested that PGRPs may play complex roles in the host immune response to bacterial pathogens in catfish.

Original languageEnglish (US)
Pages (from-to)291-299
Number of pages9
JournalDevelopmental and Comparative Immunology
Volume46
Issue number2
DOIs
StatePublished - Oct 2014
Externally publishedYes

Keywords

  • Catfish
  • Innate immunity
  • PRR
  • Pathogen recognition receptor
  • Peptidoglycan recognition protein

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

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