P-NMR analysis of the B to Z transition in double-stranded (dC-dG)3, and (dC-dG)4 in high salt solution

Tadeusz A. Holak; Philip N. Borer; George C. Levy; Jacques H. van Boom; Andrew H.J. Wang

doi:10.1093/nar/12.11.4625

P-NMR analysis of the B to Z transition in double-stranded (dC-dG)₃, and (dC-dG)₄ in high salt solution

Tadeusz A. Holak, Philip N. Borer, George C. Levy, Jacques H. van Boom, Andrew H.J. Wang

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

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Abstract

In 4H NaCl solutions (dC-dG)_n (n=3,4; ̃9mM) exist as a mixture ot B and Z forms. The low and high field components of two ³¹P NMR resonances originating from internal phosphodiester groups are assigned to the GpC and CpG linkages, respectively. Low temperatures stabilize the Z-forms, which completely disappear above 50°C (n=3) and 65°C (n=4). ΔH=-44 and -17 kJ/mol for B to Z transition in the hexamer and octamer duplexes, respectively. Temperature dependent changes (0-57°C range) in the spin-lattice relaxation times at 145.7 MHz are distinctly different for the ³¹p nuclei o± GpC and CpG groups. The relaxation data can be explained by assuming that the GpC phosphodiester groups undergo more local internal motion than do the CpG groups.

Original language	English (US)
Pages (from-to)	4625-4635
Number of pages	11
Journal	Nucleic acids research
Volume	12
Issue number	11
DOIs	https://doi.org/10.1093/nar/12.11.4625
State	Published - Jun 11 1984

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Genetics

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@article{3827810599bf40ecb0573faf580004e7,

title = "P-NMR analysis of the B to Z transition in double-stranded (dC-dG)3, and (dC-dG)4 in high salt solution",

abstract = "In 4H NaCl solutions (dC-dG)n (n=3,4;{\~ }9mM) exist as a mixture ot B and Z forms. The low and high field components of two 31P NMR resonances originating from internal phosphodiester groups are assigned to the GpC and CpG linkages, respectively. Low temperatures stabilize the Z-forms, which completely disappear above 50°C (n=3) and 65°C (n=4). ΔH=-44 and -17 kJ/mol for B to Z transition in the hexamer and octamer duplexes, respectively. Temperature dependent changes (0-57°C range) in the spin-lattice relaxation times at 145.7 MHz are distinctly different for the 31p nuclei o± GpC and CpG groups. The relaxation data can be explained by assuming that the GpC phosphodiester groups undergo more local internal motion than do the CpG groups.",

author = "Holak, {Tadeusz A.} and Borer, {Philip N.} and Levy, {George C.} and {van Boom}, {Jacques H.} and Wang, {Andrew H.J.}",

note = "Funding Information: We thank Anil Kumar for discussion and assistance with the MOLDYN calculations, Dr. M. Petersheim for supplying unpublished data and Dr. L. S. Ka.i for a preprint. This work was supported by NIHGrant GM32691 (to PNB), NIHGrants GM29778, RR01317, and NSF Grant 81-05109 (to GCL), NIH Grant CA29753 (AH-JW), and American Cancer So ;iety Grant NP 3348 (to AH-JW and A. Rich).",

year = "1984",

month = jun,

day = "11",

doi = "10.1093/nar/12.11.4625",

language = "English (US)",

volume = "12",

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journal = "Nucleic acids research",

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T1 - P-NMR analysis of the B to Z transition in double-stranded (dC-dG)3, and (dC-dG)4 in high salt solution

AU - Holak, Tadeusz A.

AU - Borer, Philip N.

AU - Levy, George C.

AU - van Boom, Jacques H.

AU - Wang, Andrew H.J.

N1 - Funding Information: We thank Anil Kumar for discussion and assistance with the MOLDYN calculations, Dr. M. Petersheim for supplying unpublished data and Dr. L. S. Ka.i for a preprint. This work was supported by NIHGrant GM32691 (to PNB), NIHGrants GM29778, RR01317, and NSF Grant 81-05109 (to GCL), NIH Grant CA29753 (AH-JW), and American Cancer So ;iety Grant NP 3348 (to AH-JW and A. Rich).

PY - 1984/6/11

Y1 - 1984/6/11

N2 - In 4H NaCl solutions (dC-dG)n (n=3,4; ̃9mM) exist as a mixture ot B and Z forms. The low and high field components of two 31P NMR resonances originating from internal phosphodiester groups are assigned to the GpC and CpG linkages, respectively. Low temperatures stabilize the Z-forms, which completely disappear above 50°C (n=3) and 65°C (n=4). ΔH=-44 and -17 kJ/mol for B to Z transition in the hexamer and octamer duplexes, respectively. Temperature dependent changes (0-57°C range) in the spin-lattice relaxation times at 145.7 MHz are distinctly different for the 31p nuclei o± GpC and CpG groups. The relaxation data can be explained by assuming that the GpC phosphodiester groups undergo more local internal motion than do the CpG groups.

AB - In 4H NaCl solutions (dC-dG)n (n=3,4; ̃9mM) exist as a mixture ot B and Z forms. The low and high field components of two 31P NMR resonances originating from internal phosphodiester groups are assigned to the GpC and CpG linkages, respectively. Low temperatures stabilize the Z-forms, which completely disappear above 50°C (n=3) and 65°C (n=4). ΔH=-44 and -17 kJ/mol for B to Z transition in the hexamer and octamer duplexes, respectively. Temperature dependent changes (0-57°C range) in the spin-lattice relaxation times at 145.7 MHz are distinctly different for the 31p nuclei o± GpC and CpG groups. The relaxation data can be explained by assuming that the GpC phosphodiester groups undergo more local internal motion than do the CpG groups.

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P-NMR analysis of the B to Z transition in double-stranded (dC-dG)₃, and (dC-dG)₄ in high salt solution

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