@article{8d52b4e7a865438191e6c9d8246ca172,
title = "Ovarian fluid proteome variation associates with sperm swimming speed in an externally fertilizing fish",
abstract = "Sperm velocity is a key trait that predicts the outcome of sperm competition. By promoting or impeding sperm velocity, females can control fertilization via postcopulatory cryptic female choice. In Chinook salmon, ovarian fluid (OF), which surrounds the ova, mediates sperm velocity according to male and female identity, biasing the outcome of sperm competition towards males with faster sperm. Past investigations have revealed proteome variation in OF, but the specific components of OF that differentially mediate sperm velocity have yet to be characterized. Here we use quantitative proteomics to investigate whether OF protein composition explains variation in sperm velocity and fertilization success. We found that OF proteomes from six females robustly clustered into two groups and that these groups are distinguished by the abundance of a restricted set of proteins significantly associated with sperm velocity. Exposure of sperm to OF from females in group I had faster sperm compared to sperm exposed to the OF of group II females. Overall, OF proteins that distinguished between these groups were enriched for vitellogenin and calcium ion interactions. Our findings suggest that these proteins may form the functional basis for cryptic female choice via the biochemical and physiological mediation of sperm velocity.",
keywords = "cryptic female choice, fertility, oocyte, sexual selection, sperm velocity",
author = "Johnson, {Sheri L.} and Kirill Borziak and Torsten Kleffmann and Patrice Rosengrave and Steve Dorus and Gemmell, {Neil J.}",
note = "Funding Information: We thank Marsha Villarroel for assistance with laboratory work. We thank the hatchery staff at Salmon Smolt, NZ, in particular Ben Divett, Karl French, Errol White, Tom Gough and Luke Price, for providing facilities, fish husbandry, gamete handling assistance and advice. We also thank Janine Wing, Tanya Blakely, Katherine McBride and Sara Ferreira for assistance in the field. This work was supported in part by grants from the Royal Society of New Zealand Marsden Fund (UOO913 to NJ), the Royal Society of New Zealand Fast‐Start Grant (UOO1209 to PR), the Eunice Shriver National Institute for Child Health and Human Development (R21‐HD088910 to SDX) and the National Science Foundation (DEB‐1655840 to SD). Funding Information: We thank Marsha Villarroel for assistance with laboratory work. We thank the hatchery staff at Salmon Smolt, NZ, in particular Ben Divett, Karl French, Errol White, Tom Gough and Luke Price, for providing facilities, fish husbandry, gamete handling assistance and advice. We also thank Janine Wing, Tanya Blakely, Katherine McBride and Sara Ferreira for assistance in the field. This work was supported in part by grants from the Royal Society of New Zealand Marsden Fund (UOO913 to NJ), the Royal Society of New Zealand Fast-Start Grant (UOO1209 to PR), the Eunice Shriver National Institute for Child Health and Human Development (R21-HD088910 to SDX) and the National Science Foundation (DEB-1655840 to SD). Publisher Copyright: {\textcopyright} 2020 European Society For Evolutionary Biology. Journal of Evolutionary Biology {\textcopyright} 2020 European Society For Evolutionary Biology",
year = "2020",
month = dec,
doi = "10.1111/jeb.13717",
language = "English (US)",
volume = "33",
pages = "1783--1794",
journal = "Journal of Evolutionary Biology",
issn = "1010-061X",
publisher = "Wiley-Blackwell",
number = "12",
}