On the modeling of endocytosis in yeast

Tao Zhang, Rastko Sknepnek, Mark John Bowick, Jennifer M Schwarz

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The cell membrane deforms during endocytosis to surround extracellular material and draw it into the cell. Results of experiments on endocytosis in yeast show general agreement that 1) actin polymerizes into a network of filaments exerting active forces on the membrane to deform it, and 2) the large-scale membrane deformation is tubular in shape. In contrast, there are three competing proposals for precisely how the actin filament network organizes itself to drive the deformation. We use variational approaches and numerical simulations to address this competition by analyzing a meso-scale model of actin-mediated endocytosis in yeast. The meso-scale model breaks up the invagination process into three stages: 1) initiation, where clathrin interacts with the membrane via adaptor proteins; 2) elongation, where the membrane is then further deformed by polymerizing actin filaments; and 3) pinch-off. Our results suggest that the pinch-off mechanism may be assisted by a pearling-like instability. We rule out two of the three competing proposals for the organization of the actin filament network during the elongation stage. These two proposals could be important in the pinch-off stage, however, where additional actin polymerization helps break off the vesicle. Implications and comparisons with earlier modeling of endocytosis in yeast are discussed.

Original languageEnglish (US)
Pages (from-to)508-519
Number of pages12
JournalBiophysical Journal
Volume108
Issue number3
DOIs
StatePublished - Feb 3 2015

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Endocytosis
Yeasts
Actin Cytoskeleton
Actins
Membranes
Clathrin
Polymerization
Cell Membrane
Proteins

ASJC Scopus subject areas

  • Biophysics

Cite this

On the modeling of endocytosis in yeast. / Zhang, Tao; Sknepnek, Rastko; Bowick, Mark John; Schwarz, Jennifer M.

In: Biophysical Journal, Vol. 108, No. 3, 03.02.2015, p. 508-519.

Research output: Contribution to journalArticle

Zhang, T, Sknepnek, R, Bowick, MJ & Schwarz, JM 2015, 'On the modeling of endocytosis in yeast', Biophysical Journal, vol. 108, no. 3, pp. 508-519. https://doi.org/10.1016/j.bpj.2014.11.3481
Zhang, Tao ; Sknepnek, Rastko ; Bowick, Mark John ; Schwarz, Jennifer M. / On the modeling of endocytosis in yeast. In: Biophysical Journal. 2015 ; Vol. 108, No. 3. pp. 508-519.
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