TY - JOUR
T1 - Olfactory horizontal basal cells demonstrate a conserved multipotent progenitor phenotype
AU - Carter, Lindsay A.
AU - MacDonald, Jessica L.
AU - Roskams, A. Jane
PY - 2004/6/23
Y1 - 2004/6/23
N2 - Stem cells of adult regenerative organs share a common goal but few established conserved mechanisms. Within the neural stem cell niche of the mouse olfactory epithelium, we identified a combination of extracellular matrix (ECM) receptors that regulate adhesion and mitosis in non-neural stem cells [intercellular adhesion molecule-1 (ICAM-1), β1, β4, and α-1, -3, and -6 integrins] and on horizontal basal cells (HBCs), candidate olfactory neuro-epithelial progenitors. Using ECM receptors as our guide, we recreated a defined microenvironment in vitro that mimics olfactory basal lamina and, when supplemented with epidermal growth factor, transforming growth factor α, and leukemia inhibitory factor, allows us to preferentially expand multiple clonal adherent colony phenotypes from individual ICAM-1 + and ICAM-1+/β1 integrin+-selected HBCs. The most highly mitotic colony-forming HBCs demonstrate multipotency, spontaneously generating more ICAM-positive presumptive HBCs, a combination of olfactory neuroglial progenitors, and neurons of olfactory and potentially nonolfactory phenotypes. HBCs thus possess a conserved adhesion receptor expression profile similar to non-neural stem cells, preferential self-replication in an in vitro environment mimicking their in vivo niche, and contain subpopulations of cells that can produce multiple differentiated neuronal and glial progeny from within and beyond the olfactory system in vitro.
AB - Stem cells of adult regenerative organs share a common goal but few established conserved mechanisms. Within the neural stem cell niche of the mouse olfactory epithelium, we identified a combination of extracellular matrix (ECM) receptors that regulate adhesion and mitosis in non-neural stem cells [intercellular adhesion molecule-1 (ICAM-1), β1, β4, and α-1, -3, and -6 integrins] and on horizontal basal cells (HBCs), candidate olfactory neuro-epithelial progenitors. Using ECM receptors as our guide, we recreated a defined microenvironment in vitro that mimics olfactory basal lamina and, when supplemented with epidermal growth factor, transforming growth factor α, and leukemia inhibitory factor, allows us to preferentially expand multiple clonal adherent colony phenotypes from individual ICAM-1 + and ICAM-1+/β1 integrin+-selected HBCs. The most highly mitotic colony-forming HBCs demonstrate multipotency, spontaneously generating more ICAM-positive presumptive HBCs, a combination of olfactory neuroglial progenitors, and neurons of olfactory and potentially nonolfactory phenotypes. HBCs thus possess a conserved adhesion receptor expression profile similar to non-neural stem cells, preferential self-replication in an in vitro environment mimicking their in vivo niche, and contain subpopulations of cells that can produce multiple differentiated neuronal and glial progeny from within and beyond the olfactory system in vitro.
KW - Degeneration
KW - Development
KW - Extracellular matrix
KW - Gliogenesis
KW - Olfactory
KW - Progenitor
UR - http://www.scopus.com/inward/record.url?scp=3042779410&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=3042779410&partnerID=8YFLogxK
U2 - 10.1523/JNEUROSCI.0330-04.2004
DO - 10.1523/JNEUROSCI.0330-04.2004
M3 - Article
C2 - 15215289
AN - SCOPUS:3042779410
SN - 0270-6474
VL - 24
SP - 5670
EP - 5683
JO - Journal of Neuroscience
JF - Journal of Neuroscience
IS - 25
ER -