Novel multistep BRET-FRET energy transfer using nanoconjugates of firefly proteins, quantum dots, and red fluorescent proteins

Rabeka Alam, Joshua Zylstra, Danielle M. Fontaine, Bruce R. Branchini, Mathew M Maye

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Sequential bioluminescence resonance energy transfer (BRET) and fluorescence resonance energy transfer (FRET) from firefly luciferase to red fluorescent proteins using quantum dot or rod acceptor/donor linkers is described. The effect of morphology and tuned optical properties on the efficiency of this unique BRET-FRET system was evaluated.

Original languageEnglish (US)
Pages (from-to)5303-5306
Number of pages4
JournalNanoscale
Volume5
Issue number12
DOIs
StatePublished - Jun 21 2013

Fingerprint

Nanoconjugates
Fireflies
Bioluminescence
Quantum Dots
Fluorescence Resonance Energy Transfer
Energy Transfer
Energy transfer
Semiconductor quantum dots
Proteins
Firefly Luciferases
Optical properties
red fluorescent protein

ASJC Scopus subject areas

  • Materials Science(all)
  • Medicine(all)

Cite this

Novel multistep BRET-FRET energy transfer using nanoconjugates of firefly proteins, quantum dots, and red fluorescent proteins. / Alam, Rabeka; Zylstra, Joshua; Fontaine, Danielle M.; Branchini, Bruce R.; Maye, Mathew M.

In: Nanoscale, Vol. 5, No. 12, 21.06.2013, p. 5303-5306.

Research output: Contribution to journalArticle

Alam, Rabeka ; Zylstra, Joshua ; Fontaine, Danielle M. ; Branchini, Bruce R. ; Maye, Mathew M. / Novel multistep BRET-FRET energy transfer using nanoconjugates of firefly proteins, quantum dots, and red fluorescent proteins. In: Nanoscale. 2013 ; Vol. 5, No. 12. pp. 5303-5306.
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