Noninvasive in vivo plasma volume and hematocrit in humans: Observing long-term baseline behavior to establish homeostasis for intravascular volume and composition

Paul Dent, Bin Deng, Jerry Goodisman, Charles M. Peterson, Sriram Narsipur, J. Chaiken

Research output: Chapter in Book/Entry/PoemConference contribution

5 Scopus citations


A new device incorporating a new algorithm and measurement process allows simultaneous noninvasive in vivo monitoring of intravascular plasma volume and red blood cell volume. The purely optical technique involves probing fingertip skin with near infrared laser light and collecting the wavelength shifted light, that is, the inelastic emission (IE) which includes the unresolved Raman and fluorescence, and the un-shifted emission, that is, the elastic emission (EE) which includes both the Rayleigh and Mie scattered light. Our excitation and detection geometry is designed so that from these two simultaneous measurements we can calculate two parameters within the single scattering regime using radiation transfer theory, the intravascular plasma volume fraction and the red blood cell volume fraction. Previously calibrated against a gold standard FDA approved device, 2 hour monitoring sessions on three separate occasions over a three week span for a specific, motionless, and mostly sleeping individual produced 3 records containing a total of 5706 paired measurements of hematocrit and plasma volume. The average over the three runs, relative to the initial plasma volume taken as 100%, of the plasma volume±1σ was 97.56±0.55 or 0.56%.For the same three runs, the average relative hematocrit (Hct), referenced to an assumed initial value of 28.35 was 29.37±0.12 or stable to ±0.4%.We observe local deterministic circulation effects apparently associated with the pressure applied by the finger probe as well as longer timescale behavior due to normal ebb and flow of internal fluids due to posture changes and tilt table induced gravity gradients.

Original languageEnglish (US)
Title of host publicationBiophotonics
Subtitle of host publicationPhotonic Solutions for Better Health Care V
EditorsValery V. Tuchin, Francesco S. Pavone, Jurgen Popp, Dennis L. Matthews
ISBN (Electronic)9781510601321
StatePublished - 2016
EventBiophotonics: Photonic Solutions for Better Health Care V - Brussels, Belgium
Duration: Apr 4 2016Apr 7 2016

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X


OtherBiophotonics: Photonic Solutions for Better Health Care V


  • Binary spectronephelometry
  • Fluorescence
  • Hematocrit
  • Noninvasive blood analysis
  • Plasma volume
  • Raman

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


Dive into the research topics of 'Noninvasive in vivo plasma volume and hematocrit in humans: Observing long-term baseline behavior to establish homeostasis for intravascular volume and composition'. Together they form a unique fingerprint.

Cite this