Non-contact Raman spectroscopic pH measurement of cerebrospinal fluid: In vivo rat and perimortem swine models

Seth Fillioe, Kyle Kelly Bishop, Josh Satalin, Sarah Blair, Charles M. Peterson, Gary Nieman, Alexander Vincent Struck Jannini, Jon Jong In Kim, Richard McDonough, Steven Ortiz, Jerry Goodisman, Julie Hasenwinkel, J. Chaiken

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We recently reported the first noninvasive, label free measurement of pH in a bodily fluid in vivo using only Raman spectra i.e. in vivo rat model measurements probing the immediate vicinity of a contusive spinal cord injury (SCI) in the first minutes and hours after injury. Calibrated and assigned using Raman spectra of authentic materials, in the rat model we were not able to sample the cerebrospinal fluid (CSF) to allow comparison with an independent measurement of the pH. Swine presents a better model because they allow physical sampling of CSF, although still not ideal for our purposes. We were only able to physically sample CSF from the fourth cerebral ventricle of 2 different animals, before and after all spectral measurements on cords were completed. One measurement each for 2 different animals on physically sampled CSF averaged a pH of 7.001±0.106 (N=2) as per standard laboratory instrumentation. Using a dynamic analysis and the Henderson-Hasselbalch equation, the average of (N=12) noninvasive Raman-based pH measurements of CSF was 7.073±0.156 and at >95% confidence there is no statistically significant difference between the Raman-based and the physically sampled results. We discuss the difference between the dynamic and static analysis, the implications for our understanding of SCI, the accuracy, precision, calibration, general applicability of this approach and future work.

Original languageEnglish (US)
Title of host publicationPhotonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2020
EditorsTianhong Dai, Jurgen Popp, Mei X. Wu
PublisherSPIE
ISBN (Electronic)9781510632097
DOIs
StatePublished - Jan 1 2020
EventPhotonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2020 - San Francisco, United States
Duration: Feb 3 2020Feb 4 2020

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume11223
ISSN (Print)1605-7422

Conference

ConferencePhotonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2020
CountryUnited States
CitySan Francisco
Period2/3/202/4/20

Keywords

  • In vivo
  • Inflammation
  • Noninvasive
  • PV[O]H
  • Physiological monitoring
  • Raman
  • Spinal cord injury
  • pH

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Fillioe, S., Bishop, K. K., Satalin, J., Blair, S., Peterson, C. M., Nieman, G., Jannini, A. V. S., Kim, J. J. I., McDonough, R., Ortiz, S., Goodisman, J., Hasenwinkel, J., & Chaiken, J. (2020). Non-contact Raman spectroscopic pH measurement of cerebrospinal fluid: In vivo rat and perimortem swine models. In T. Dai, J. Popp, & M. X. Wu (Eds.), Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2020 [1122308] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11223). SPIE. https://doi.org/10.1117/12.2546415