NMR studies on structural features of DNA [d(TCGCGCG)]2 with a dangling residue T

Ke Yu Wang, You Jie Zhang, Philip N. Borer

Research output: Contribution to journalArticle

Abstract

The DNA heptamer [d(TCGCGCG)]2, which has an alternating pyrimidine-purine sequence and a dangling T residue at the 5′- end has been used as a model for studying conformational characteristics of DNA duplexes with double-single strand junctions that occur during replication and transcription. The proton resonances of the molecule have been assigned by NOESY and TOCSY/HOHAHA experiments. The NOE intensities at seven different mixing times were quantified for calculating distance constraints with the two-spin approximation. The experiments have uncovered the interesting structural features of the molecule. The sugar proton coupling constants were determined by the P. E. COSY experiment for each residue of the duplex to examine the sugar conformations of the DNA heptamer. The structural features of the heptamer in solution have been obtained. The overall structure of the DNA duplex is basically of a B-type DNA, and substantial changes from a standard B-DNA have been observed, especially in the terminal region of the molecule. The dangling T residue was found in an unusual syn-conformation.

Original languageEnglish (US)
Pages (from-to)1177-1184
Number of pages8
JournalScience in China (Scientia Sinica) Series B
Volume37
Issue number10
StatePublished - Oct 1 1994

Keywords

  • 2d NMR
  • DAN conformation
  • [d(TCGCGCG)]

ASJC Scopus subject areas

  • Environmental Science(all)
  • Engineering(all)
  • Earth and Planetary Sciences(all)

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