Next generation sequencing yields the complete mitochondrial genome of the striped raphael catfish, Platydoras armatulus (Siluriformes

Doradidae)

Shikai Liu, Jun Yao, Jiaren Zhang, Zhanjian "John" Liu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Striped raphael catfish, Platydoras armatulus, belongs to catfish family Doradidae, which are endemic to South America and featured by a single row of thorny scutes along the lateral line. In this study, we determined the complete mitochondrial genome of P. armatulus by next generation sequencing of genomic DNA without prior sample processing or primer design. Bioinformatics analysis provided the full-length mitogenome sequence of 16,470 bp. The P. armatulus mitogenome is composed of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and 1 control region, showing typical circular molecule structure as in other vertebrates. The whole genome base composition was estimated to be 30.9% A, 25.2% T, 15.9% G and 28.0% C, with A/T bias of 56.1%. The generation of P. armatulus mitogenome sequence enriched catfish molecular resources that are valuable for phylogenetic analysis and species identification studies.

Original languageEnglish (US)
Pages (from-to)1963-1964
Number of pages2
JournalMitochondrial DNA. Part A, DNA mapping, sequencing, and analysis
Volume27
Issue number3
DOIs
StatePublished - May 1 2016
Externally publishedYes

Fingerprint

Catfishes
Mitochondrial Genome
South America
Base Composition
Transfer RNA
Computational Biology
DNA Sequence Analysis
rRNA Genes
Vertebrates
Genome
Genes
Proteins

Keywords

  • Mitochondrial genome
  • next generation sequencing
  • Platydoras armatulus
  • striped raphael catfish

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

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title = "Next generation sequencing yields the complete mitochondrial genome of the striped raphael catfish, Platydoras armatulus (Siluriformes: Doradidae)",
abstract = "Striped raphael catfish, Platydoras armatulus, belongs to catfish family Doradidae, which are endemic to South America and featured by a single row of thorny scutes along the lateral line. In this study, we determined the complete mitochondrial genome of P. armatulus by next generation sequencing of genomic DNA without prior sample processing or primer design. Bioinformatics analysis provided the full-length mitogenome sequence of 16,470 bp. The P. armatulus mitogenome is composed of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and 1 control region, showing typical circular molecule structure as in other vertebrates. The whole genome base composition was estimated to be 30.9{\%} A, 25.2{\%} T, 15.9{\%} G and 28.0{\%} C, with A/T bias of 56.1{\%}. The generation of P. armatulus mitogenome sequence enriched catfish molecular resources that are valuable for phylogenetic analysis and species identification studies.",
keywords = "Mitochondrial genome, next generation sequencing, Platydoras armatulus, striped raphael catfish",
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AU - Liu, Shikai

AU - Yao, Jun

AU - Zhang, Jiaren

AU - Liu, Zhanjian "John"

PY - 2016/5/1

Y1 - 2016/5/1

N2 - Striped raphael catfish, Platydoras armatulus, belongs to catfish family Doradidae, which are endemic to South America and featured by a single row of thorny scutes along the lateral line. In this study, we determined the complete mitochondrial genome of P. armatulus by next generation sequencing of genomic DNA without prior sample processing or primer design. Bioinformatics analysis provided the full-length mitogenome sequence of 16,470 bp. The P. armatulus mitogenome is composed of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and 1 control region, showing typical circular molecule structure as in other vertebrates. The whole genome base composition was estimated to be 30.9% A, 25.2% T, 15.9% G and 28.0% C, with A/T bias of 56.1%. The generation of P. armatulus mitogenome sequence enriched catfish molecular resources that are valuable for phylogenetic analysis and species identification studies.

AB - Striped raphael catfish, Platydoras armatulus, belongs to catfish family Doradidae, which are endemic to South America and featured by a single row of thorny scutes along the lateral line. In this study, we determined the complete mitochondrial genome of P. armatulus by next generation sequencing of genomic DNA without prior sample processing or primer design. Bioinformatics analysis provided the full-length mitogenome sequence of 16,470 bp. The P. armatulus mitogenome is composed of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and 1 control region, showing typical circular molecule structure as in other vertebrates. The whole genome base composition was estimated to be 30.9% A, 25.2% T, 15.9% G and 28.0% C, with A/T bias of 56.1%. The generation of P. armatulus mitogenome sequence enriched catfish molecular resources that are valuable for phylogenetic analysis and species identification studies.

KW - Mitochondrial genome

KW - next generation sequencing

KW - Platydoras armatulus

KW - striped raphael catfish

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