Negative effect of the 5'-untranslated leader sequence on Ac transposon promoter expression

Katia Castanho Scortecci, Ramesh Raina, Nina V. Fedoroff, Marie Anne Van Sluys

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Transposable elements are used in heterologous plant hosts to clone genes by insertional mutagenesis. The Activator (Ac) transposable element has been cloned from maize, and introduced into a variety of plants. However, differences in regulation and transposition frequency have been observed between different host plants. The cause of this variability is still unknown. To better understand the activity of the Ac element, we analyzed the Ac promoter region and its 5'-untranslated leader sequence (5' UTL). Transient assays in tobacco NT1 suspension cells showed that the Ac promoter is a weak promoter and its activity was localized by deletion analyses. The data presented here indicate that the core of the Ac promoter is contained within 153 bp fragment upstream to transcription start sites. An important inhibitory effect (80%) due to the presence of the 5' UTL was found on the expression of LUC reporter gene. Here we demonstrate that the presence of the 5' UTL in the constructs reduces the expression driven by either strong or weak promoters.

Original languageEnglish (US)
Pages (from-to)935-944
Number of pages10
JournalPlant Molecular Biology
Volume40
Issue number6
DOIs
StatePublished - Aug 1999
Externally publishedYes

Fingerprint

DNA Transposable Elements
transposons
Genes
promoter regions
Mutagenesis
Tobacco
Transcription Initiation Site
Genetic Promoter Regions
Assays
Suspensions
Insertional Mutagenesis
Reporter Genes
host plants
Zea mays
insertional mutagenesis
Clone Cells
transposition (genetics)
reporter genes
cell suspension culture
tobacco

Keywords

  • 5' UTL region
  • Ac transposon
  • Inhibitory effect
  • Promoter activity
  • Transient assays

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Negative effect of the 5'-untranslated leader sequence on Ac transposon promoter expression. / Scortecci, Katia Castanho; Raina, Ramesh; Fedoroff, Nina V.; Van Sluys, Marie Anne.

In: Plant Molecular Biology, Vol. 40, No. 6, 08.1999, p. 935-944.

Research output: Contribution to journalArticle

Scortecci, Katia Castanho ; Raina, Ramesh ; Fedoroff, Nina V. ; Van Sluys, Marie Anne. / Negative effect of the 5'-untranslated leader sequence on Ac transposon promoter expression. In: Plant Molecular Biology. 1999 ; Vol. 40, No. 6. pp. 935-944.
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