Near infrared Raman spectroscopic study of reactive gliosis and the glial scar in injured rat spinal cords

Tarun Saxena, Bin Deng, Eric Lewis-Clark, Kyle Hoellger, Dennis Stelzner, Julie Hasenwinkel, Joseph Chaiken

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

Comparative Raman spectra of ex vivo, saline-perfused, injured and healthy rat spinal cord as well as experiments using enzymatic digestion suggest that proteoglycan over expression may be observable in injured tissue. Comparison with authentic materials in vitro suggest the occurrence of side reactions between products of cord digestion with chondroitinase (cABC) that produce lactones and similar species with distinct Raman features that are often not overlapped with Raman features from other chemical species. Since the glial scar is thought to be a biochemical and physical barrier to nerve regeneration, this observation suggests the possibility of using near infrared Raman spectroscopy to study disease progression and explore potential treatments ex vivo and if potential treatments can be designed, perhaps to monitor potential remedial treatments within the spinal cord in vivo.

Original languageEnglish (US)
Title of host publicationBiomedical Vibrational Spectroscopy IV
Subtitle of host publicationAdvances in Research and Industry
DOIs
StatePublished - May 7 2010
EventBiomedical Vibrational Spectroscopy IV: Advances in Research and Industry - San Francisco, CA, United States
Duration: Jan 23 2010Jan 25 2010

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7560
ISSN (Print)1605-7422

Other

OtherBiomedical Vibrational Spectroscopy IV: Advances in Research and Industry
CountryUnited States
CitySan Francisco, CA
Period1/23/101/25/10

Keywords

  • Chondroitin sulfate proteoglycans
  • Chondroitinase ABC
  • Glial scar
  • Raman
  • Spinal cord injury

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Saxena, T., Deng, B., Lewis-Clark, E., Hoellger, K., Stelzner, D., Hasenwinkel, J., & Chaiken, J. (2010). Near infrared Raman spectroscopic study of reactive gliosis and the glial scar in injured rat spinal cords. In Biomedical Vibrational Spectroscopy IV: Advances in Research and Industry [75600I] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7560). https://doi.org/10.1117/12.846897