Near infrared Raman spectroscopic study of reactive gliosis and the glial scar in injured rat spinal cords

Tarun Saxena, Bin Deng, Eric Lewis-Clark, Kyle Hoellger, Dennis Stelzner, Julie M Hasenwinkel, Joseph Chaiken

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Comparative Raman spectra of ex vivo, saline-perfused, injured and healthy rat spinal cord as well as experiments using enzymatic digestion suggest that proteoglycan over expression may be observable in injured tissue. Comparison with authentic materials in vitro suggest the occurrence of side reactions between products of cord digestion with chondroitinase (cABC) that produce lactones and similar species with distinct Raman features that are often not overlapped with Raman features from other chemical species. Since the glial scar is thought to be a biochemical and physical barrier to nerve regeneration, this observation suggests the possibility of using near infrared Raman spectroscopy to study disease progression and explore potential treatments ex vivo and if potential treatments can be designed, perhaps to monitor potential remedial treatments within the spinal cord in vivo.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7560
DOIs
StatePublished - 2010
EventBiomedical Vibrational Spectroscopy IV: Advances in Research and Industry - San Francisco, CA, United States
Duration: Jan 23 2010Jan 25 2010

Other

OtherBiomedical Vibrational Spectroscopy IV: Advances in Research and Industry
CountryUnited States
CitySan Francisco, CA
Period1/23/101/25/10

Fingerprint

spinal cord
scars
Gliosis
Neuroglia
rats
Cicatrix
Rats
Digestion
Spinal Cord
Chondroitinases and Chondroitin Lyases
Infrared radiation
Nerve Regeneration
Architectural Accessibility
Near-Infrared Spectroscopy
Raman Spectrum Analysis
Lactones
Proteoglycans
Disease Progression
nerves
regeneration

Keywords

  • Chondroitin sulfate proteoglycans
  • Chondroitinase ABC
  • Glial scar
  • Raman
  • Spinal cord injury

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Saxena, T., Deng, B., Lewis-Clark, E., Hoellger, K., Stelzner, D., Hasenwinkel, J. M., & Chaiken, J. (2010). Near infrared Raman spectroscopic study of reactive gliosis and the glial scar in injured rat spinal cords. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7560). [75600I] https://doi.org/10.1117/12.846897

Near infrared Raman spectroscopic study of reactive gliosis and the glial scar in injured rat spinal cords. / Saxena, Tarun; Deng, Bin; Lewis-Clark, Eric; Hoellger, Kyle; Stelzner, Dennis; Hasenwinkel, Julie M; Chaiken, Joseph.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7560 2010. 75600I.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Saxena, T, Deng, B, Lewis-Clark, E, Hoellger, K, Stelzner, D, Hasenwinkel, JM & Chaiken, J 2010, Near infrared Raman spectroscopic study of reactive gliosis and the glial scar in injured rat spinal cords. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7560, 75600I, Biomedical Vibrational Spectroscopy IV: Advances in Research and Industry, San Francisco, CA, United States, 1/23/10. https://doi.org/10.1117/12.846897
Saxena T, Deng B, Lewis-Clark E, Hoellger K, Stelzner D, Hasenwinkel JM et al. Near infrared Raman spectroscopic study of reactive gliosis and the glial scar in injured rat spinal cords. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7560. 2010. 75600I https://doi.org/10.1117/12.846897
Saxena, Tarun ; Deng, Bin ; Lewis-Clark, Eric ; Hoellger, Kyle ; Stelzner, Dennis ; Hasenwinkel, Julie M ; Chaiken, Joseph. / Near infrared Raman spectroscopic study of reactive gliosis and the glial scar in injured rat spinal cords. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7560 2010.
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