Near infrared bioluminescence resonance energy transfer from firefly luciferase - Quantum dot bionanoconjugates

Rabeka Alam; Liliana M. Karam; Tennyson L. Doane; Joshua Zylstra; Danielle M. Fontaine; Bruce R. Branchini; Mathew M. Maye

doi:10.1088/0957-4484/25/49/495606

Near infrared bioluminescence resonance energy transfer from firefly luciferase - Quantum dot bionanoconjugates

Rabeka Alam, Liliana M. Karam, Tennyson L. Doane, Joshua Zylstra, Danielle M. Fontaine, Bruce R. Branchini, Mathew M. Maye

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

The bioluminescence resonance energy transfer (BRET) between firefly luciferase enzymes and semiconductive quantum dots (QDs) with near infrared emission is described. The QD were phase transferred to aqueous buffers using a histidine mediated phase transfer route, and incubated with a hexahistidine tagged, green emitting variant of firefly luciferase from Photinus pyralis (PPyGRTS). The PPyGRTS were bound to the QD interface via the hexahistidine tag, which effectively displaces the histidine layer and binds directly to the QD interfaces, allowing for short donor-acceptor distances (∼5.5 nm). Due to this, high BRET efficiency ratios of ∼5 were obtained. These PPyGRTS-QD bio-nano conjugates were characterized by transmission electron microscopy, thermal gravimetric analysis, Fourier transform infrared spectroscopy and BRET emission studies. The final optimized conjugate was easily observable by night vision imaging, demonstrating the potential of these materials in imaging and signaling/sensing applications.

Original language	English (US)
Article number	495606
Journal	Nanotechnology
Volume	25
Issue number	49
DOIs	https://doi.org/10.1088/0957-4484/25/49/495606
State	Published - Dec 12 2014

Keywords

FRET
bioluminescence
quantum dot
resonance energy transfer
self-assembly

ASJC Scopus subject areas

Bioengineering
General Chemistry
General Materials Science
Mechanics of Materials
Mechanical Engineering
Electrical and Electronic Engineering

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10.1088/0957-4484/25/49/495606

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title = "Near infrared bioluminescence resonance energy transfer from firefly luciferase - Quantum dot bionanoconjugates",

abstract = "The bioluminescence resonance energy transfer (BRET) between firefly luciferase enzymes and semiconductive quantum dots (QDs) with near infrared emission is described. The QD were phase transferred to aqueous buffers using a histidine mediated phase transfer route, and incubated with a hexahistidine tagged, green emitting variant of firefly luciferase from Photinus pyralis (PPyGRTS). The PPyGRTS were bound to the QD interface via the hexahistidine tag, which effectively displaces the histidine layer and binds directly to the QD interfaces, allowing for short donor-acceptor distances (∼5.5 nm). Due to this, high BRET efficiency ratios of ∼5 were obtained. These PPyGRTS-QD bio-nano conjugates were characterized by transmission electron microscopy, thermal gravimetric analysis, Fourier transform infrared spectroscopy and BRET emission studies. The final optimized conjugate was easily observable by night vision imaging, demonstrating the potential of these materials in imaging and signaling/sensing applications.",

keywords = "FRET, bioluminescence, quantum dot, resonance energy transfer, self-assembly",

author = "Rabeka Alam and Karam, {Liliana M.} and Doane, {Tennyson L.} and Joshua Zylstra and Fontaine, {Danielle M.} and Branchini, {Bruce R.} and Maye, {Mathew M.}",

note = "Publisher Copyright: {\textcopyright} 2014 IOP Publishing Ltd.",

year = "2014",

month = dec,

day = "12",

doi = "10.1088/0957-4484/25/49/495606",

language = "English (US)",

volume = "25",

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T1 - Near infrared bioluminescence resonance energy transfer from firefly luciferase - Quantum dot bionanoconjugates

AU - Alam, Rabeka

AU - Karam, Liliana M.

AU - Doane, Tennyson L.

AU - Zylstra, Joshua

AU - Fontaine, Danielle M.

AU - Branchini, Bruce R.

AU - Maye, Mathew M.

PY - 2014/12/12

Y1 - 2014/12/12

N2 - The bioluminescence resonance energy transfer (BRET) between firefly luciferase enzymes and semiconductive quantum dots (QDs) with near infrared emission is described. The QD were phase transferred to aqueous buffers using a histidine mediated phase transfer route, and incubated with a hexahistidine tagged, green emitting variant of firefly luciferase from Photinus pyralis (PPyGRTS). The PPyGRTS were bound to the QD interface via the hexahistidine tag, which effectively displaces the histidine layer and binds directly to the QD interfaces, allowing for short donor-acceptor distances (∼5.5 nm). Due to this, high BRET efficiency ratios of ∼5 were obtained. These PPyGRTS-QD bio-nano conjugates were characterized by transmission electron microscopy, thermal gravimetric analysis, Fourier transform infrared spectroscopy and BRET emission studies. The final optimized conjugate was easily observable by night vision imaging, demonstrating the potential of these materials in imaging and signaling/sensing applications.

AB - The bioluminescence resonance energy transfer (BRET) between firefly luciferase enzymes and semiconductive quantum dots (QDs) with near infrared emission is described. The QD were phase transferred to aqueous buffers using a histidine mediated phase transfer route, and incubated with a hexahistidine tagged, green emitting variant of firefly luciferase from Photinus pyralis (PPyGRTS). The PPyGRTS were bound to the QD interface via the hexahistidine tag, which effectively displaces the histidine layer and binds directly to the QD interfaces, allowing for short donor-acceptor distances (∼5.5 nm). Due to this, high BRET efficiency ratios of ∼5 were obtained. These PPyGRTS-QD bio-nano conjugates were characterized by transmission electron microscopy, thermal gravimetric analysis, Fourier transform infrared spectroscopy and BRET emission studies. The final optimized conjugate was easily observable by night vision imaging, demonstrating the potential of these materials in imaging and signaling/sensing applications.

KW - FRET

KW - bioluminescence

KW - quantum dot

KW - resonance energy transfer

KW - self-assembly

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Near infrared bioluminescence resonance energy transfer from firefly luciferase - Quantum dot bionanoconjugates

Abstract

Keywords

ASJC Scopus subject areas

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