Natural diversity in daily rhythms of gene expression contributes to phenotypic variation

Amaury De Montaigu, Antonis Giakountis, Matthew Rubin, Réka Tóth, Frédéric Cremer, Vladislava Sokolova, Aimone Porri, Matthieu Reymond, Cynthia Weinig, George Coupland

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

Daily rhythms of gene expression provide a benefit to most organisms by ensuring that biological processes are activated at the optimal time of day. Although temporal patterns of expression control plant traits of agricultural importance, how natural genetic variation modifies these patterns during the day and how precisely these patterns influence phenotypes is poorly understood. The circadian clock regulates the timing of gene expression, and natural variation in circadian rhythms has been described, but circadian rhythms are measured in artificial continuous conditions that do not reflect the complexity of biologically relevant day/ night cycles. By studying transcriptional rhythms of the eveningexpressed gene GIGANTEA (GI) at high temporal resolution and during day/night cycles, we show that natural variation in the timing of GI expression occurs mostly under long days in 77 Arabidopsis accessions. This variation is explained by natural alleles that alter light sensitivity of GI, specifically in the evening, and that act at least partly independent of circadian rhythms. Natural alleles induce precise changes in the temporal waveformof GI expression, and these changes have detectable effects on PHYTOCHROME INTERACTING FACTOR 4 expression and growth. Our findings provide a paradigm for how natural alleles act within day/night cycles to precisely modify temporal gene expression waveforms and cause phenotypic diversity. Such alleles could confer an advantage by adjusting the activity of temporally regulated processes without severely disrupting the circadian system.

Original languageEnglish (US)
Pages (from-to)905-910
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume112
Issue number3
DOIs
StatePublished - Jan 20 2015

Keywords

  • Arabidopsis
  • Circadian
  • Diurnal
  • GIGANTEA
  • Rhythms

ASJC Scopus subject areas

  • General

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