Myosin filament assembly onto myofibrils in live neonatal cardiomyocytes observed by TPEF-SHG microscopy

Honghai Liu; Yonghong Shao; Wan Qin; Raymond B. Runyan; Meifeng Xu; Zhen Ma; Thomas K. Borg; Roger Markwald; Bruce Z. Gao

doi:10.1093/cvr/cvs328

Myosin filament assembly onto myofibrils in live neonatal cardiomyocytes observed by TPEF-SHG microscopy

Honghai Liu, Yonghong Shao, Wan Qin, Raymond B. Runyan, Meifeng Xu, Zhen Ma, Thomas K. Borg, Roger Markwald, Bruce Z. Gao

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

AimsUnderstanding myofibrillogenesis is essential for elucidating heart muscle formation, development, and remodelling in response to physiological stimulation. Here, we report the dynamic assembly process of contractile myosin filaments onto myofibrils in a live cardiomyocyte culture during myofibrillogenesis.Methods and resultsUtilizing a custom-built, two-photon excitation fluorescence and second harmonic generation imaging system equipped with an on-stage incubator, we observed new sarcomere additions in rat neonatal cardiomyocytes during 10 h of on-stage incubation. The new sarcomere additions occurred at the side of existing myofibrils, where we observed mature myofibrils acting as templates, or at the interstice of several separated myofibrils.ConclusionsDuring sarcomeric addition, myosin filaments are assembled onto the premyofibril laterally. This lateral addition, which proceeds stepwise along the axial direction, plays an important role in the accumulation of Z-bodies to form mature Z-disks and in the regulation of sarcomeric alignment during maturation.

Original language	English (US)
Pages (from-to)	262-270
Number of pages	9
Journal	Cardiovascular Research
Volume	97
Issue number	2
DOIs	https://doi.org/10.1093/cvr/cvs328
State	Published - Feb 1 2013
Externally published	Yes

Keywords

Myofibrillogenesis
Myosin filaments
On-stage incubator
Sarcomere addition
Second harmonic generation

ASJC Scopus subject areas

General Medicine

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10.1093/cvr/cvs328

Cite this

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title = "Myosin filament assembly onto myofibrils in live neonatal cardiomyocytes observed by TPEF-SHG microscopy",

abstract = "AimsUnderstanding myofibrillogenesis is essential for elucidating heart muscle formation, development, and remodelling in response to physiological stimulation. Here, we report the dynamic assembly process of contractile myosin filaments onto myofibrils in a live cardiomyocyte culture during myofibrillogenesis.Methods and resultsUtilizing a custom-built, two-photon excitation fluorescence and second harmonic generation imaging system equipped with an on-stage incubator, we observed new sarcomere additions in rat neonatal cardiomyocytes during 10 h of on-stage incubation. The new sarcomere additions occurred at the side of existing myofibrils, where we observed mature myofibrils acting as templates, or at the interstice of several separated myofibrils.ConclusionsDuring sarcomeric addition, myosin filaments are assembled onto the premyofibril laterally. This lateral addition, which proceeds stepwise along the axial direction, plays an important role in the accumulation of Z-bodies to form mature Z-disks and in the regulation of sarcomeric alignment during maturation.",

keywords = "Myofibrillogenesis, Myosin filaments, On-stage incubator, Sarcomere addition, Second harmonic generation",

author = "Honghai Liu and Yonghong Shao and Wan Qin and Runyan, {Raymond B.} and Meifeng Xu and Zhen Ma and Borg, {Thomas K.} and Roger Markwald and Gao, {Bruce Z.}",

note = "Funding Information: This work was supported by National Institute of Health (P20RR021949 and 1k25hl088262-01); National Science Foundation (MRI CBET-0923311 and SC EPSCoR RII EPS-0903795 through SC GEAR program); The National Natural Science Foundation of China (31171372); and the Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province (Shenzen University).",

year = "2013",

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day = "1",

doi = "10.1093/cvr/cvs328",

language = "English (US)",

volume = "97",

pages = "262--270",

journal = "Cardiovascular Research",

issn = "0008-6363",

publisher = "Oxford University Press",

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T1 - Myosin filament assembly onto myofibrils in live neonatal cardiomyocytes observed by TPEF-SHG microscopy

AU - Liu, Honghai

AU - Shao, Yonghong

AU - Qin, Wan

AU - Runyan, Raymond B.

AU - Xu, Meifeng

AU - Ma, Zhen

AU - Borg, Thomas K.

AU - Markwald, Roger

AU - Gao, Bruce Z.

N1 - Funding Information: This work was supported by National Institute of Health (P20RR021949 and 1k25hl088262-01); National Science Foundation (MRI CBET-0923311 and SC EPSCoR RII EPS-0903795 through SC GEAR program); The National Natural Science Foundation of China (31171372); and the Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province (Shenzen University).

PY - 2013/2/1

Y1 - 2013/2/1

N2 - AimsUnderstanding myofibrillogenesis is essential for elucidating heart muscle formation, development, and remodelling in response to physiological stimulation. Here, we report the dynamic assembly process of contractile myosin filaments onto myofibrils in a live cardiomyocyte culture during myofibrillogenesis.Methods and resultsUtilizing a custom-built, two-photon excitation fluorescence and second harmonic generation imaging system equipped with an on-stage incubator, we observed new sarcomere additions in rat neonatal cardiomyocytes during 10 h of on-stage incubation. The new sarcomere additions occurred at the side of existing myofibrils, where we observed mature myofibrils acting as templates, or at the interstice of several separated myofibrils.ConclusionsDuring sarcomeric addition, myosin filaments are assembled onto the premyofibril laterally. This lateral addition, which proceeds stepwise along the axial direction, plays an important role in the accumulation of Z-bodies to form mature Z-disks and in the regulation of sarcomeric alignment during maturation.

AB - AimsUnderstanding myofibrillogenesis is essential for elucidating heart muscle formation, development, and remodelling in response to physiological stimulation. Here, we report the dynamic assembly process of contractile myosin filaments onto myofibrils in a live cardiomyocyte culture during myofibrillogenesis.Methods and resultsUtilizing a custom-built, two-photon excitation fluorescence and second harmonic generation imaging system equipped with an on-stage incubator, we observed new sarcomere additions in rat neonatal cardiomyocytes during 10 h of on-stage incubation. The new sarcomere additions occurred at the side of existing myofibrils, where we observed mature myofibrils acting as templates, or at the interstice of several separated myofibrils.ConclusionsDuring sarcomeric addition, myosin filaments are assembled onto the premyofibril laterally. This lateral addition, which proceeds stepwise along the axial direction, plays an important role in the accumulation of Z-bodies to form mature Z-disks and in the regulation of sarcomeric alignment during maturation.

KW - Myofibrillogenesis

KW - Myosin filaments

KW - On-stage incubator

KW - Sarcomere addition

KW - Second harmonic generation

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Myosin filament assembly onto myofibrils in live neonatal cardiomyocytes observed by TPEF-SHG microscopy

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