Myofibrillogenesis in live neonatal cardiomyocytes observed with hybrid two-photon excitation fluorescence-second harmonic generation microscopy

Honghai Liu; Wan Qin; Yonghong Shao; Zhen Ma; Tong Ye; Tom Borg; Bruce Z. Gao

doi:10.1117/1.3662457

Myofibrillogenesis in live neonatal cardiomyocytes observed with hybrid two-photon excitation fluorescence-second harmonic generation microscopy

Honghai Liu, Wan Qin, Yonghong Shao, Zhen Ma, Tong Ye, Tom Borg, Bruce Z. Gao

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

We developed a hybrid two-photon excitation fluorescence-second harmonic generation (TPEF-SHG) imaging system with an on-stage incubator for long-term live-cell imaging. Using the imaging system, we observed the addition of new sarcomeres during myofibrillogenesis while a cardiomyocyte was spreading on the substrate. The results suggest that the TPEF-SHG imaging system with an on-stage incubator is an effective tool for investigation of dynamic myofibrillogenesis.

Original language	English (US)
Article number	126012
Journal	Journal of biomedical optics
Volume	16
Issue number	12
DOIs	https://doi.org/10.1117/1.3662457
State	Published - Dec 2011
Externally published	Yes

Keywords

Cardiomyocytes
Myofibrillogenesis
On-stage incubator
Sarcomere addition
Second harmonic generation
Two-photon excitation fluorescence

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Biomedical Engineering

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10.1117/1.3662457

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@article{ffb97e4a1803435bb7a765371b56cffa,

title = "Myofibrillogenesis in live neonatal cardiomyocytes observed with hybrid two-photon excitation fluorescence-second harmonic generation microscopy",

abstract = "We developed a hybrid two-photon excitation fluorescence-second harmonic generation (TPEF-SHG) imaging system with an on-stage incubator for long-term live-cell imaging. Using the imaging system, we observed the addition of new sarcomeres during myofibrillogenesis while a cardiomyocyte was spreading on the substrate. The results suggest that the TPEF-SHG imaging system with an on-stage incubator is an effective tool for investigation of dynamic myofibrillogenesis.",

keywords = "Cardiomyocytes, Myofibrillogenesis, On-stage incubator, Sarcomere addition, Second harmonic generation, Two-photon excitation fluorescence",

author = "Honghai Liu and Wan Qin and Yonghong Shao and Zhen Ma and Tong Ye and Tom Borg and Gao, {Bruce Z.}",

note = "Funding Information: This work has been partially supported by NIH (SC COBRE P20RR0 949 and Career Award 1k25hl088262–01), NSF (MRI CBET-0923311 and SC EPSCoR RII EPS-0903795 through SC GEAR program), The National Natural Science Foundation of China (31171372), Guangdong Province Science and Technology Project (10B060300002) and Shenzhen University Application Technology Development Project (201136). We acknowledge Dr. Jeffrey Holmes for valuable discussions. BZG would also like to acknowledge support from the grant established by the State Key Laboratory of Precision Measuring Technology and Instruments (Tianjin University).",

year = "2011",

month = dec,

doi = "10.1117/1.3662457",

language = "English (US)",

volume = "16",

journal = "Journal of biomedical optics",

issn = "1083-3668",

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number = "12",

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TY - JOUR

T1 - Myofibrillogenesis in live neonatal cardiomyocytes observed with hybrid two-photon excitation fluorescence-second harmonic generation microscopy

AU - Liu, Honghai

AU - Qin, Wan

AU - Shao, Yonghong

AU - Ma, Zhen

AU - Ye, Tong

AU - Borg, Tom

AU - Gao, Bruce Z.

N1 - Funding Information: This work has been partially supported by NIH (SC COBRE P20RR0 949 and Career Award 1k25hl088262–01), NSF (MRI CBET-0923311 and SC EPSCoR RII EPS-0903795 through SC GEAR program), The National Natural Science Foundation of China (31171372), Guangdong Province Science and Technology Project (10B060300002) and Shenzhen University Application Technology Development Project (201136). We acknowledge Dr. Jeffrey Holmes for valuable discussions. BZG would also like to acknowledge support from the grant established by the State Key Laboratory of Precision Measuring Technology and Instruments (Tianjin University).

PY - 2011/12

Y1 - 2011/12

N2 - We developed a hybrid two-photon excitation fluorescence-second harmonic generation (TPEF-SHG) imaging system with an on-stage incubator for long-term live-cell imaging. Using the imaging system, we observed the addition of new sarcomeres during myofibrillogenesis while a cardiomyocyte was spreading on the substrate. The results suggest that the TPEF-SHG imaging system with an on-stage incubator is an effective tool for investigation of dynamic myofibrillogenesis.

AB - We developed a hybrid two-photon excitation fluorescence-second harmonic generation (TPEF-SHG) imaging system with an on-stage incubator for long-term live-cell imaging. Using the imaging system, we observed the addition of new sarcomeres during myofibrillogenesis while a cardiomyocyte was spreading on the substrate. The results suggest that the TPEF-SHG imaging system with an on-stage incubator is an effective tool for investigation of dynamic myofibrillogenesis.

KW - Cardiomyocytes

KW - Myofibrillogenesis

KW - On-stage incubator

KW - Sarcomere addition

KW - Second harmonic generation

KW - Two-photon excitation fluorescence

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AN - SCOPUS:84860585937

SN - 1083-3668

VL - 16

JO - Journal of biomedical optics

JF - Journal of biomedical optics

IS - 12

M1 - 126012

ER -

Myofibrillogenesis in live neonatal cardiomyocytes observed with hybrid two-photon excitation fluorescence-second harmonic generation microscopy

Abstract

Keywords

ASJC Scopus subject areas

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