Myofibrillogenesis in live neonatal cardiomyocytes observed with hybrid two-photon excitation fluorescence-second harmonic generation microscopy

Honghai Liu, Wan Qin, Yonghong Shao, Zhen Ma, Tong Ye, Tom Borg, Bruce Z. Gao

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

We developed a hybrid two-photon excitation fluorescence-second harmonic generation (TPEF-SHG) imaging system with an on-stage incubator for long-term live-cell imaging. Using the imaging system, we observed the addition of new sarcomeres during myofibrillogenesis while a cardiomyocyte was spreading on the substrate. The results suggest that the TPEF-SHG imaging system with an on-stage incubator is an effective tool for investigation of dynamic myofibrillogenesis.

Original languageEnglish (US)
Article number126012
JournalJournal of Biomedical Optics
Volume16
Issue number12
DOIs
StatePublished - Dec 2011
Externally publishedYes

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Keywords

  • Cardiomyocytes
  • Myofibrillogenesis
  • On-stage incubator
  • Sarcomere addition
  • Second harmonic generation
  • Two-photon excitation fluorescence

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

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