Multiple across-strain and within-strain QTLs suggest highly complex genetic architecture for hypoxia tolerance in channel catfish

Xiaozhu Wang, Shikai Liu, Chen Jiang, Xin Geng, Tao Zhou, Ning Li, Lisui Bao, Yun Li, Jun Yao, Yujia Yang, Xiaoxiao Zhong, Yulin Jin, Rex Dunham, Zhanjian "John" Liu

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The ability to survive hypoxic conditions is important for various organisms, especially for aquatic animals. Teleost fish, representing more than 50 % of vertebrate species, are extremely efficient in utilizing low levels of dissolved oxygen in water. However, huge variations exist among various taxa of fish in their ability to tolerate hypoxia. In aquaculture, hypoxia tolerance is among the most important traits because hypoxia can cause major economic losses. Genetic enhancement for hypoxia tolerance in catfish is of great interest, but little was done with analysis of the genetic architecture of hypoxia tolerance. The objective of this study was to conduct a genome-wide association study to identify QTLs for hypoxia tolerance using the catfish 250K SNP array with channel catfish families from six strains. Multiple significant and suggestive QTLs were identified across and within strains. One significant QTL and four suggestive QTLs were identified across strains. Six significant QTLs and many suggestive QTLs were identified within strains. There were rare overlaps among the QTLs identified within the six strains, suggesting a complex genetic architecture of hypoxia tolerance. Overall, within-strain QTLs explained larger proportion of phenotypic variation than across-strain QTLs. Many of genes within these identified QTLs have known functions for regulation of oxygen metabolism and involvement in hypoxia responses. Pathway analysis indicated that most of these genes were involved in MAPK or PI3K/AKT/mTOR signaling pathways that were known to be important for hypoxia-mediated angiogenesis, cell proliferation, apoptosis and survival.

Original languageEnglish (US)
Pages (from-to)63-76
Number of pages14
JournalMolecular Genetics and Genomics
Volume292
Issue number1
DOIs
StatePublished - Feb 1 2017
Externally publishedYes

Fingerprint

Ictaluridae
Catfishes
Genetic Enhancement
Fishes
Oxygen
Hypoxia
Aquatic Organisms
Aquaculture
Genome-Wide Association Study
Phosphatidylinositol 3-Kinases
Genes
Single Nucleotide Polymorphism
Vertebrates
Cell Survival
Economics
Cell Proliferation
Apoptosis

Keywords

  • Climate change
  • Fish
  • GWAS
  • Hypoxia tolerance
  • Oxygen
  • QTL

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Multiple across-strain and within-strain QTLs suggest highly complex genetic architecture for hypoxia tolerance in channel catfish. / Wang, Xiaozhu; Liu, Shikai; Jiang, Chen; Geng, Xin; Zhou, Tao; Li, Ning; Bao, Lisui; Li, Yun; Yao, Jun; Yang, Yujia; Zhong, Xiaoxiao; Jin, Yulin; Dunham, Rex; Liu, Zhanjian "John".

In: Molecular Genetics and Genomics, Vol. 292, No. 1, 01.02.2017, p. 63-76.

Research output: Contribution to journalArticle

Wang, X, Liu, S, Jiang, C, Geng, X, Zhou, T, Li, N, Bao, L, Li, Y, Yao, J, Yang, Y, Zhong, X, Jin, Y, Dunham, R & Liu, ZJ 2017, 'Multiple across-strain and within-strain QTLs suggest highly complex genetic architecture for hypoxia tolerance in channel catfish', Molecular Genetics and Genomics, vol. 292, no. 1, pp. 63-76. https://doi.org/10.1007/s00438-016-1256-2
Wang, Xiaozhu ; Liu, Shikai ; Jiang, Chen ; Geng, Xin ; Zhou, Tao ; Li, Ning ; Bao, Lisui ; Li, Yun ; Yao, Jun ; Yang, Yujia ; Zhong, Xiaoxiao ; Jin, Yulin ; Dunham, Rex ; Liu, Zhanjian "John". / Multiple across-strain and within-strain QTLs suggest highly complex genetic architecture for hypoxia tolerance in channel catfish. In: Molecular Genetics and Genomics. 2017 ; Vol. 292, No. 1. pp. 63-76.
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