Molecular characterization and gene expression of the channel catfish ferritin H subunit after bacterial infection and iron treatment

Hong Liu, Tomokazu Takano, Eric Peatman, Jason Abernathy, Shaolin Wang, Zhenxia Sha, Huseyin Kucuktas, De Hai Xu, Phillip Klesius, Zhanjian "John" Liu

Research output: Contribution to journalArticle

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Abstract

Ferritins are the major iron storage protein in the cytoplasm of cells, responsible for regulating levels of intracellular iron. Ferritin genes are widely distributed in both prokaryotes and eukaryotes. In mammals, ferritin molecules are composed of heavy-(H) and light-(L) chain subunits; amphibian genomes contain three ferritin-type genes (H; middle, M; and L subunits); and teleost genomes to date contain H and M subunits. The objective of this study was to characterize the ferritin H gene in channel catfish (Ictalurus punctatus) to determine its genomic organization and copy numbers, to determine its patterns of tissue expression, and to establish if it is involved in defense responses of catfish after bacterial infection. The catfish ferritin H gene was completely sequenced and characterized, using both mRNA and genomic DNA. Catfish ferritin H gene has a full-length mRNA sequence of 999bp, an open reading frame of 534bp, and 4,704bp genomic DNA sequence. Catfish ferritin H has a 5 exon and 4 intron genetic organization, containing a long 50-untranslated region, which shares high similarity with mammalian and zebrafish genes. Based on phylogenetic analyses, the catfish ferritin H gene is highly conserved throughout evolution. Southern blot analysis suggested that the ferritin H gene has only one copy in the catfish genome. The catfish ferritin H gene was widely expressed in various healthy tissues. The catfish ferritin H gene was significantly up-regulated in the liver after intraperitoneal injection of iron dextran and coinjection of Edwardsiella ictaluri and iron dextran treatment, suggesting its role in iron metabolism and immunity.

Original languageEnglish (US)
Pages (from-to)359-368
Number of pages10
JournalJournal of Experimental Zoology Part A: Ecological Genetics and Physiology
Volume313 A
Issue number6
DOIs
StatePublished - Jul 1 2010
Externally publishedYes

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ASJC Scopus subject areas

  • Physiology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Animal Science and Zoology
  • Genetics

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