Modified critical angle method for measuring the refractive index of bio-optical materials and its application to bacteriorhodopsin

Q. Wang Song, Chin Yu Ku, Chunping Zhang, Richard B. Gross, Robert R. Birge, Richard Michalak

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

The critical angle technique is modified for the accurate measurement of the refractive index of bio-optical materials. Based on the analysis of reflection from the boundary of the material as a function of incident angle and polarization direction, the critical illumination angle is obtained by numerical differentiation of the reflection curve. As an example, the dispersion curve of bacteriorhodopsin is given. The measurement error and the effect of the host bovine skin gelatin on the results are analyzed.

Original languageEnglish (US)
Pages (from-to)797-803
Number of pages7
JournalJournal of the Optical Society of America B: Optical Physics
Volume12
Issue number5
DOIs
StatePublished - Jan 1995

ASJC Scopus subject areas

  • Statistical and Nonlinear Physics
  • Atomic and Molecular Physics, and Optics

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